葛根素和淫羊藿甙对成骨细胞生物学作用的比较研究  

Comparative Research of Biological Effect of Puerarin and Icariine on Osteoblast Cells

作　　者：殷晓雪[1] 陈仲强[1] 党耕町[1] 

机构地区：[1]北京大学第三医院骨科,北京市100083

出　　处：《中国骨与关节损伤杂志》2007年第3期210-212,共3页Chinese Journal of Bone and Joint Injury

摘　　要：目的对比葛根素和淫羊藿甙对成骨细胞增殖和分化的影响。方法将小鼠骨髓基质干细胞定向诱导分化为成骨细胞,取第3、4代细胞,以rhBMP-2为阳性对照,MTT法观察葛根素和淫羊藿甙对成骨细胞的增殖作用,碱性磷酸酶(ALP)比活性测定及钙结节计数观察葛根素和淫羊藿甙对成骨细胞分化的影响。结果20μg/ml的淫羊藿甙可显著促进成骨细胞的增殖;20μg/ml淫羊藿甙及50μg/ml葛根素均能提高成骨细胞的碱性磷酸酶活性,并使矿化结节的形成明显增多。结论淫羊藿甙不仅能促进成骨细胞的分化,还能增强其增殖能力,而葛根素主要是通过刺激分化来增强成骨细胞的活力。Objective To study comparatively the biological effect of Puerarin and Icariine on the proliferation and differentiation of osteoblasts. Methods The osteoblasts were obtained by inducting mice mesenchymal stem cells （hMSCs） directionally. MTT was used to observe the proliferation and activity of ALP, mineralized nodule was assayed to observe the differentiation of osteoblasts cultured in vitro. Results The 20μg/ml Icariine increased greatly the proliferation of osteoblasts. The 20 μg/ml Icariine and 50 μg/ml Puerarin increased greatly the activity of ALP and the amount of mineralized nodule. Conclusion Icariine has the effect of increasing the prolif, eration and differentiation of osteoblasts while Puerarin mainly stimulates the differentiation of osteoblasts.

关 键 词：葛根素 淫羊藿甙 成骨细胞 对比研究 

分 类 号：R681[医药卫生—骨科学]