巨细胞病毒感染致人脐静脉内皮细胞E选择素时序性的表达  

Sequential changes of E-selectin mRNA in human umbilical vein endothelial cells with human cytomegalovirus infection

作　　者：刘真真[1] 周亚滨[1] 唐伟[1] 李树英[1] 吴建敏[1] 程轶喆[1] 齐眉[1] 王红[1] 刘娟[1] 于晗[1] 

机构地区：[1]实验畸形学教育部重点实验室山东大学医学院微生物学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第2期124-126,共3页Journal of Shandong University:Health Sciences

基　　金：山东大学大学生科技创新基金项目

摘　　要：目的:观察人类巨细胞病毒(human cytomegalovirus,HCMV)作用于血管内皮细胞(vein endothelialcells,ECV)对E选择素时序性的表达,探讨HCMV感染致动脉粥样硬化(atherosclerosis,AS)发生和发展的可能机制。方法:用HCMV感染ECV,采用RT-PCR方法检测不同感染时段细胞E选择素mRNA及蛋白的表达。结果:HCMV感染ECV后,感染0 h时E选择素mRNA有基础水平的表达,4 h升高并达高峰,8 h时开始回落,12 h回落显著,24 h回落更明显,与0 h比较,差异均有统计学意义(P<0.01),48 h接近0 h表达水平(P>0.05)。结论:HCMV感染ECV后能够促进E选择素表达,并且呈时间依赖性。HCMV有可能通过上调E选择素的表达,介导ECV的炎症反应,促进AS的发生、发展。Objective： By investigating sequential changes of E-selectin in human umbilical vein endothelial cells （HUVEC） by human cytomegalovirus （HCMV） infection, to study the potential mechanism of atheroscleresis（AS） induced by cytomegalovirus infection. Methods： HUVEC was cultured and then infected by HCMV. Expression of E-selectin mRNA was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results： Four hours after cytomegalovirus infection, the expression of E-selectin increasel. As the time of infection went by, the expression of E-selectin gradually decreased. When the cytomegalovirus infection lasted 48 hours, the expression of E-selectin returned to the fundamental level. There were significant differences among the groups at 0, 4, 8, 12, 24 and 48 hours （ P 〈 0.01 ）. Conclusions： The infection with HCMV can enhance the expression of E-selectin mRNA in HUVEC. So we can say that HCMV may induce the inflammatory reaction by up-regulating the E-selectin expression on endothelial cells, which facilitates the occurrence and development of AS.

关 键 词：人类巨细胞病毒 人脐静脉内皮细胞 E选择素 

分 类 号：R373[医药卫生—病原生物学]