甘蓝型油菜pol CMS育性恢复基因的分子标记  

Identification of genetic markers for the pol CMS restorer gene in Brassica napus L.

作　　者：刘平武[1] 李赟[1] 何庆彪[1] 杨光圣[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室国家油菜改良武汉分中心,湖北武汉430070

出　　处：《中国油料作物学报》2007年第1期14-19,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家973项目(2001CB1088);国家863重大专项(2002AA207009);长江学者和创新团队发展计划资助(IRT0442);华中农业大学科研启动项目

摘　　要：以甘蓝型油菜pol CMS不育系1141A及其恢复系花叶恢为亲本构建F2分离群体,并进一步构建可育和不育分离群体集团。利用RAPD、SSR、AFLP等技术进行标记筛选,获得与Rfp基因连锁的2个分子标记,这2个标记分布于Rfp的两侧,其中,AFLP标记E7P16230与Rfp遗传图距最近,为4.3cM;另一侧的RAPD标记S1-500与Rfp遗传图距为10.8cM。RAPD, SSR and AFLP technology combined with bulked segregant analysis were performed to identify genetic markers for pol CMS restorer gene Rfp and to map the markers using an F2 population of a pol CMS line 1141A crossed to its restorer line Huayehui. One AFLP marker E7P16230 and one RAPD marker S1 -500 were identified linked to Rfp locus. These two genetic markers flank Rfp gene on different sides, and closely linked to Rfp with a genetic distance of 4.3 cM for E7P16230 and 10.8 cM for S1 - 500, respectively.

关 键 词：甘蓝型油菜 细胞质雄性不育 育性恢复基因 分子标记 

分 类 号：S565.403.3[农业科学—作物学]