杂色鲍♀×盘鲍♂杂交受精的细胞学研究  

Cytological Studies on the Fertilization of the Cross Between Haliotis disversicolor♀ and H.discus discus♂

作　　者：蔡明夷[1] 柯才焕[1] 游伟伟[1] 王桂忠[1] 王志勇[2] 王艺磊[2] 

机构地区：[1]厦门大学海洋学系,近海海洋环境科学国家重点实验室(厦门大学),福建厦门361005 [2]集美大学水产生物技术研究所,福建厦门361021

出　　处：《厦门大学学报（自然科学版）》2007年第2期239-243,共5页Journal of Xiamen University：Natural Science

基　　金：国家863计划课题(2003AA603240)资助

摘　　要：应用组织学方法,研究了杂色鲍♀×盘鲍♂种间杂交受精过程的核相变化,结果表明杂色鲍♀×盘鲍♂种间杂交受精主要表现出两性融合的特点.盘鲍精子进入杂色鲍卵子,9 min后中心粒发育为星光;精子头部逐渐膨大、液化形成雄性原核,39 min后与雌性原核会合,而后融合.约3.2%的受精卵中观察到1个受精卵中同时存在3个即将融合的原核,这可能是多精入卵或卵子染色体组自发加倍的结果.42 min后开始第1次有丝分裂,此时在极个别发育卵中发现固缩的染色质小体、微核、或分离不同步的染色体.To obtain useful information about the genetic essence of the progenies of the interspecific cross between the small abalone （Haliotis disversicolor） ♀ and the Japanese abalone （H. discus discus） ♂ ,nuclear behavior in hybrid eggs was examined during meiosis and first cleavage under a light microscope. The results showed that the nucleus in hybrid eggs behaved like those in amphimictic eggs. After the sperm of the Japanese abalone incorporated into the egg cytoplasm of the small abalone,the sperm nucleus gradually dispersed and developed into the male pronucleus. The female pronucleus eventually merged with the female pronucleus,since that few dense chromatin bodies were observed. Tripronucleus was observed in bout 3.2 % zygotes, which may result from polyspermy or spontaneous diploidization of maternal chromosome set. At anaphase of mitosis I, some abnormal phenomena were observed in several eggs,such as asynchronous segregation of chromosome and formation of micronuclei.

关 键 词：种间杂交 受精细胞学 杂色鲍 盘鲍 雌核发育 

分 类 号：Q32[生物学—遗传学] Q959.215