脉络宁抗新生鼠缺氧缺血性脑损伤作用及其机制探讨  

Protective Effect and Mechanism of Mailuoning on Hypoxic-ischemic Brain Damage in Neonatal Rats

作　　者：孙克娅 张留宝 蔡萤 蒋正生[1] 任智红[1] 

机构地区：[1]无锡市儿童医院急诊科,214001 [2]金陵药业股份有限公司,南京210009 [3]南京军医学院,210000

出　　处：《国际儿科学杂志》2007年第2期83-86,共4页International Journal of Pediatrics

摘　　要：目的 研究新生SD鼠缺氧缺血后大脑皮质谷氨酸和天冬氨酸的变化，观察脑神经元型一氧化氮合酶（nNOS）和c-fos蛋白的表达，探讨脉络宁对缺氧缺血性脑损伤（HIBO）的保护作用。方法 结扎新生7日龄SD大鼠右侧颈总动脉1h（缺血1h），吸入8％氧和92％氮2h（缺氧2h），建立HIBD模型。用色谱分析仪测定大脑皮质内谷氨酸和天冬氨酸含量，用免疫组化方法动态评价缺氧缺血和脉络宁治疗不同时相c-fos蛋白和nNOS的表达。结果 缺氧缺血后大脑皮质兴奋性氨基酸（ZAA）水平升高，经脉络宁处理后则明显降低。c-fos蛋白表达的高峰期呈现在缺氧缺血6h后，与缺氧缺血组相比，脉络宁处理后c-fos蛋白表达的脑神经元数目增多；与假手术组相比，缺氧缺血可上调nNOS的表达水平，脉络宁处理后则减少了nNOS的表达。结论 脉络宁通过改善神经元功能减少EAA的释放，调节nNOS的免疫活性，对神经元起保护作用。脉络宁可增强和延长神经元内c-fos蛋白的表达，减轻HIBD，推测c-fos参与了缺氧缺血后大脑皮质和海马神经元损伤后的修复、存活与再生。Objective To detect changes of aspartate（ASP）and glutamaic acid（GLU）in cerebral cortex of neonatal Sprague-Dawely（SD）rats after hypoxia-ischemia（HI）and observe the expression of nitric oxide synthase（ NOS ） and protein e-fos in cerebral neurons of neonatal SD rats. Mailuoning＇s protective effect onhypoxia-ischemia brain damage （HIBD）was explored. Methods The HIBD models were established as follows. The fight common carotid arteries of the neonatal SD rats in seven days old were temporarily ligatured for 1 h, then the neonatal SD rats were exposed to 8% oxygen and 92% nitrogen gas mixture for 2h. The ASP and GLU were determined in fight cerebral cortex by chromatograph and the expression of c-fos and NOS were dynamically evaluated by immunohistochemistry in different periods in neonatal SD rats after HI and Mailuoning administrated. Results The level of excitatory amino acids was promoted in fight cerebral cortex after hypoxia-ischemia. The volume of excitatory amino acid was reduced sharply after Mailuoning＇s administration.The expression of c-fos reached the peak after hypoxia-ischemia for six hours. As compared with the hypoxic-ischemic group, the numberer c-fos positive neurons increased and the expressive time of c-Fos prolonged in Mailuoning administrated group. As compared with the false-operation group, the level of NOS expression in cerebral neurons elevated after HI, while it decreased in the Mailuoning administrated group. Conclusions Mailuoning may adjust immunologieal activity of NOS in cerebral neurons properly and protect the neurons after hypoxia-ischemia through supplying blood to neurons and reducing release of excitatory amino acid,while Mailuoning may enhance and prolong the expression of c-fos ineerebral neurons after HI as well as reduce HIBD. So it is inferred that c-fos participate in the repair,survival and regeneration of neurons in cerebral cortex and hippocampus after HI.

关 键 词：缺氧缺血 脑 一氧化氮合酶 中草药 

分 类 号：R28[医药卫生—中药学]