水稻品种RGA分析与抗瘟性鉴定  

Genetic Diversity and Resistance Identification among Rice Varieties Based on Resistance Gene Analogue in Yunnan Province

作　　者：李晔[1] 范静华[2] 何月秋[2] 朱有勇[2] 

机构地区：[1]沈阳大学环境工程重点实验室,辽宁沈阳110044 [2]云南农业大学云南省植物病理重点实验室,云南昆明650201

出　　处：《江西农业大学学报》2007年第1期11-15,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家863计划资助项目(编号:2001AA211091)

摘　　要：根据抗病基因在保守区域序列同源的原理,利用RGA方法对云南省主要栽培品种和地方资源品种的遗传多样性进行了分析。从22个水稻品种的抗病基因同源序列中,共扩增出155条谱带,各品种之间谱带较清晰呈现明显的多态性,聚类分析结果可以明显将品种的抗感水平分开,也与温室人工接种试验结果相似。因此,利用RGA分析可以为水稻品种抗瘟性鉴定提供一定的理论依据。The genetic diversity of the commercial and local resource cultivars of rice in Yunnan Province was studied using resistance gene analogue （RGA） based on resistance gene conserved sequence. The results showed that both the primer pairs were identified with polymorphism, which produced 155 amplification bands. The varieties were genetically highly diverse and completely distinguished from RGA analysis. The result of clustering analysis was similar to the one of inoculation in greenhouse. The RGA analysis provided the theoretical basis for the resistance identification of rice variety.

关 键 词：水稻 抗病基因同源序列分析 多态性 抗性鉴定 

分 类 号：S511.034[农业科学—作物学]