雪柑试管苗不定根高频率诱导和高效再生体系的建立  

High Efficiency Induction of Adventitious Roots and in Vitro Regeneration System of Sweet Orange 'Xuegan'

作　　者：徐海峰[1] 栾爱业[1] 曾黎辉[1] 吴少华[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002

出　　处：《江西农业大学学报》2007年第1期148-151,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：教育部留学回国人员科研启动基金;福建省自然科学基金(B0510018);福建省教育厅科技项目(JA03062)资助

摘　　要：以雪柑实生苗上胚轴为材料,建立了高效离体培养再生体系。上胚轴纵切结合前期暗培养,在MS附加6-BA 3mg/L、蔗糖30g/L培养基中,不定芽诱导率为100%,每个外植体平均诱导的不定芽数达33.8;不定芽在1 000mg/L IBA溶液中浸泡10min后,移入1/4MT(大量元素)+1/2MT(其它元素)+10g/L蔗糖的液体培养基中,采用滤纸桥法进行培养,生根率达100%,平均根数2.5条;移苗成活率达100%。High efficiency in vitro regeneration system of sweet orange＇ Xuegan＇ was developed using epicotyl segments from in vitro seedlings as explants. Epicotyls were cured longitudinally and cultured in the dark first, on the MS basal medium with 3 mg/L 6 - BA and 30 g/L sugar, 100% adventitious shoot induction and an average of 33.8 shoots per explant were obtained. The base of the adventitious shoots was treated with 1000 mg/L IBA for 10 min, then placed onto the filter papers which ends dipped into liquid medium with 1/4 MT （macro -inorganic salts）, 1/2 MT（other inorganic salts and vitamins） and 1 g/L sugar. 100% rooting and an average of 2.5 roots per shoot were achieved. Plantlets 100% adapted well to soil conditions.

关 键 词：雪柑 上胚轴 不定根诱导 再生体系 离体培养 

分 类 号：S666.4[农业科学—果树学]