组织工程骨修复牙槽嵴裂的实验研究  

An investigation of restoration of alveolar cleft with engineered bone

作　　者：欧新荣[1] 翦新春[1] 林戈 

机构地区：[1]中南大学湘雅医院,口腔医学院口腔颌面外科,长沙410078 [2]国家人类干细胞工程技术研究中心

出　　处：《中华整形外科杂志》2007年第1期29-31,共3页Chinese Journal of Plastic Surgery

摘　　要：目的探讨骨髓来源的种子细胞复合胶原蛋白海绵构建组织工程骨修复牙槽嵴裂的可行性。方法12条实验犬被分成4组。于双侧上颌第3切牙处去除长15mm牙槽骨形成牙槽嵴裂动物模型；经股骨骨髓穿刺，分离骨髓基质干细胞，培养、传代扩增诱导后，与蛋白胶原海绵混合培养48h，植入骨缺损处。饲养12周后处死动物，通过三维CT及组织学检查评价骨缺损修复的效果。结果实验组牙槽骨断端间形成完整的骨连接，切片可见髓腔通畅，新形成的牙槽嵴宽度与松质骨组相似（P〉0．05），但高度不足（P〈0．05）。结论组织工程骨可以较好地修复牙槽嵴裂，有望成为修复牙槽嵴裂的治疗方法。Objective To investigate restoration of alveolar cleft with engineered bone constructed by sponge collagen protein combined bone mesenchymal stem cells （BMSC）. Methods Twelve dogs were divided into 4 groups, the third incisor and alveolar bone with periosteum in bilateral maxilla were removed to form alveolar cleft model. The BMSCs were isolated from dog bone marrow. After being cultured and induced, the BMSCs were seeded in sponge collagen protein and cultured for 48 hours. The composites of BMSCs and collagen were implanted into the defect of alveolar cleft. After 12 weeks＇ feeding, those dogs were sacrificed. Threedimensional CT and histological examination were used to observe the progress of bone formation. Results The defects healed at 12 weeks after being implant BMSCs-collagen composites, the width of engineered bone is resembled with positive control, but the height is less than positive control. Conclusions The engineered bone can restore the defect of alveolar bone effectively, it can be used clinically to treat alveolar cleft.

关 键 词：组织工程骨 牙槽嵴裂 修复 

分 类 号：R686[医药卫生—骨科学]