自体骨髓基质干细胞复合珊瑚修复犬下颌骨节段缺损的初步研究  

Pilot study of ruing autologons bone marrow stromal cells and coral to repair canine segmental mandibular defects

作　　者：袁捷[1] 刘广鹏[1] 柴岗[1] 刘波[1] 许锋[1] 崔磊[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海市组织工程研究重点实验室,200011

出　　处：《中华整形外科杂志》2007年第1期51-55,共5页Chinese Journal of Plastic Surgery

基　　金：国家“973”组织工程基本科学问题项目（G1999054308）;国家“863”组织工程化骨构建技术研究与产品开发（2002AA2050011）;上海市教委“重中之重”重点学科资助;国家自然科学基金项目（30600650）

摘　　要：目的应用自体骨髓基质干细胞（bone marrow stromal cells，BMSCs）复合珊瑚构建组织工程化骨，修复犬下颌骨节段性缺损。方法体外扩增培养、成骨诱导犬BMSCs。将第二代细胞复合珊瑚后修复犬自体右侧3cm的下颌骨节段缺损（n=6）；以单纯珊瑚植入缺损处为对照（n=6），术后12、32周分别通过影像学，大体形态观察，组织学和生物力学的方法检测骨缺损的修复效果。结果成骨诱导的BMSCs在珊瑚支架上生长良好。X线片显示12周时实验组骨痂较多，对照组材料明显吸收；32周时CT、X线片和大体观察显示术后实验组骨愈合良好，对照组为骨不连；骨密度检测示实验组显著高于对照组（P〈0．05）；组织学示实验组有较多成熟骨呈骨性愈合，对照组为纤维性愈合；生物力学测试实验组与正常下颌骨力学强度差异无统计学意义（P〉0．05）。结论自体成骨诱导BMSCs复合珊瑚形成的组织工程化骨可修复犬下颌骨节段缺损。Objective To repair segmental mandibular defects with autologous bone marrow stromal cells （BMSCs） engineered bone. Methods Isolated BMSCs were expanded in vitro and osteogenic induced. In 12 canines, a 3 cm segmental mandibular defect at fight mandible was created. 6 canine＇s defects were repaired with cell-scaffold constructs made from induced BMSCs and coral; others were repaired with coral as control. The engineered bone was evaluated by X-ray, CT, Dual Energy X-ray Absorptiometry （DXA）, gross and histological examination, and biomechanical test pest-operatively. Results Induced BMSCs grew well on coral scaffold. At 12 weeks, X-ray showed more callus formed in experimental group, while evident scaffold degration in control group. At 32 weeks, gross observation, X-ray and CT demonstrated well bony-union in experimental group, while bony-nonunion in control group. Also DXA revealed significantly higher bone mineral density of experimental group than control group. Histologically, mature bone were commonly observed and there were bony healing in experimental group, while fibrous healing occured in control group. Biomechanical test revealed no significant difference between experimental group and normal group. Conclusions Canine segmental mandibular defects can be repaired with the tissue-engineered bone generated by coral scaffold with autologous osteogenic BMSCs.

关 键 词：下颌骨 骨髓基质干细胞 珊瑚 组织工程 

分 类 号：R686[医药卫生—骨科学]