GPA基因突变与慢性苯中毒的关系  

Association of glycophorin A gene mutation in peripheral erythrocytes and chronic benzene poisoning

作　　者：邢彩虹[1] 纪之莹[1] 李桂兰[1] 尹松年[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《中华劳动卫生职业病杂志》2007年第2期87-90,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨外周血红细胞血型糖蛋白A（glycophorin A，GPA）基因突变与慢性苯中毒的关系。方法用荧光标记单克隆抗体和流式细胞术，检测63名慢性苯中毒工人和45名同期接苯但无苯中毒表现的同工种对照工人外周血红细胞GPA基因突变频率。结果苯中毒组的白细胞、中性粒细胞计数明显低于对照组，差异有统计学意义（P〈0．01）；随着GPA-NN、GPA-Nφ突变频率增加，白细胞及中性粒细胞计数相应减少，两者间的趋势关系均有统计学意义（P〈0．05）。GPA-NN突变频率随着累积接触评分增加而增加，两者间的趋势关系有统计学意义（P〈0．05）。GPA-Nφ突变频率与累积接触评分间的趋势关系不明显（P〉0．05）。结论苯中毒所表现的外周血白细胞、中性粒细胞数的降低，可能来源于骨髓干细胞的遗传损伤，即GPA-MN基因的纯合型（NN）基因突变。GPA-NN基因突变在慢性苯中毒的发病过程中起着重要作用。Objective To explore the association of the glycophorin A（GPA） gene mutation in peripheral erythrocytes and chronic benzene poisoning. Methods Sixty-three patients with chronic benzene poisoning and 45 benzene-exposed workers who were engaged in the same job title were investigated. Fluorescence immunolabeling technique and flowcytometry were used to detect GPA mutation frequency in peripheral read blood cell. Results A significant decrease in WBC count and neutrophil count was found in patients with chronic benzene poisoning compared with control individuals （P〈0.01）. The WBC count and neutrophil count both decreased along with the GPA-NN frequency, and the trends were significant （P〈0.05）. Both WBC counts and neutrophil counts decreased as the frequency, and trends were significant （P〈0.05）. GPA-NN frequency increased along with the accumulative exposure score, and the trend was significant （P=0.0026）. There was no significant trend between the GPA-Nφ frequency and the accumulative exposure score （P=0.2037）. Conclusion A decrease in WBC count and neutrophil count is found in patients with chronic benzene poisoning, which can arise from genetic damage in bone marrow stem cells, namely gene- duplicating mutations CNN） at the GPA locus in bone marrow cells of MN-heterozygous subjects, GPA-NN mutagens contributed to the pathogenesis of chronic benzene poisoning.

关 键 词：苯 中毒 基因 GPA 

分 类 号：R686[医药卫生—骨科学]