妊娠相关血浆蛋白-A的纯化与鉴定  

Purification and Identification of Pregnancy-associated Plasma Protein A

作　　者：顾荣花[1] 孙丽洲[1] 陈湘健[1] 顾春荣[1] 卞智萍[1] 徐晋丹[1] 杨笛[2] 张寄南[2] 

机构地区：[1]南京医科大学第一附属医院妇产科,江苏南京210029 [2]南京医科大学第一附属医院心血管病研究所,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第2期167-170,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省高新技术研究项目(BG2003033);江苏省医学重点实验室基金(SK200205);江苏省高技术重点实验室基金(BM2002202)资助

摘　　要：目的:建立人妊娠血浆相关蛋白A(PAPP-A)的纯化与鉴定方法。方法:利用DEAE-Sephadex CL-6B离子交换和Heparin-sepharose CL-6B亲和层析进行分离纯化,变性SDS-PAGE测定其相对分子质量为200kD,Western blotting鉴定其特异性。结果:获得相对分子质量为200kD的蛋白条带,此条带可与妊娠血浆相关蛋白A单克隆抗体发生特异性反应。结论:成功地从孕妇血清中分离得到妊娠血浆相关蛋白A。Objective：To develop a method for the purification and identification of human Pregnancy-associated plasma protein A. Methods：Pregnancy-associated Plasma Protein A fraction was isolated and purified by DEAE-Sepharose CL-6B ion-exchange, and heparin-Sepharose CI-6B affinity chromatography, denatured SDS-PAGE and Western blot method respectively. Results：The expressed protein, with a relative molecular weight of 200 kD, showed specific reaction with McAb to pregnancy-associated plasma protein A. Conclusion：Pregnancy-associated plasma protein A could be successfully isolated from pregnancy serum.

关 键 词：妊娠血浆相关蛋白A 纯化 鉴定 

分 类 号：TQ937[化学工程]