不同程度、类型脊髓损伤后活性氧水平的变化  

Changes of ROS Level in Different Types and Degrees of Spinal Cord Injury

作　　者：陈光学[1] 王廷华[1] 敖丽娟[2] 於建鹏 胡忆[4] 陆地[1] 

机构地区：[1]昆明医学院神经科学研究所,云南昆明650031 [2]昆明医学院第二附属医院康复医学科,云南昆明650101 [3]黄石市第一人民医院麻醉科,湖北黄石435001 [4]昆明医学院科研处,云南昆明650031

出　　处：《昆明医学院学报》2007年第1期1-4,8,共5页Journal of Kunming Medical College

基　　金：国家自然科学基金资助(30560170);昆明医学院中青年基金资助(03041)

摘　　要：目的研究脊髓损伤(SCI)后脊髓组织内丙二醛(MDA)和超氧化物岐化酶(SOD)的含量变化,以探讨活性氧(ROS)在脊髓继发性损伤中的作用机制.方法30只健康雌性成年SD大鼠采用改良式Allen氏法和脊髓横截面切割法分别造成50 cmg、25 cmg挫伤和半、全横截面切割伤四组及对照组模型,测定造模后6 h损伤组和对照组脊髓组织内MDA和SOD含量并各组之间相互作比较.结果SCI后6 h,四种损伤组与对照组比较MDA含量都明显升高(P<0.05),SOD活性都显著下降(P<0.05);在同一类型SCI的不同程度之间比较除半、全横截面切割伤之间SOD活性差别显著(P<0.05)外,其余都显示无显著性的差异(P>0.05).结论不同程度和类型的SCI后脊髓组织内ROS水平明显增高,因而说明在脊髓继发性损伤的病理过程中ROS可能是一种重要的参与者;同一类型的SCI脊髓组织的ROS水平在不同程度损伤模型之间比较,ROS含量与损伤程度并非完全呈正相关.Objective To observe the changes of malonyldialdehyed （MDA） and superoxide dissmutas （SOD） contents in spinal cord after two different types and degrees of spinal cord injury （SCI） alad to study the reactive oxygen species variation in secondary pathological damage of the spinal cord. Methods Thirty Sprague - Dawley rats were randomly divided into five groups： control group, 50 cmg and 25 cmg contusion groups, hemi - section and transection injury groups. The SCI models in rats were made with modified Allen＇ s method and intersection cutting. The amount of MDA and SOD of spinal cord tissue were determined in 6h after injury. Results The concentration of MDA significantly increased and the activity of SOD significantly descended in all the types of SCI in 6 h （P 〈0. 05）. No statistically significant difference was seen regarding MDA and SOD contents in the groups of the same type of SCI （P 〉0. 05） except the SOD in the hemi -section and transection injury groups （P 〉0. 05）. Conclusions The reactive oxygen species （ROS） plays a crucial role in the secondary pathological damage of SCI. The level of ROS in the injured spinal cord tissue is not completely consistent with degree of injury.

关 键 词：脊髓损伤 活性氧 丙二醛 超氧化物岐化酶 

分 类 号：R651.2[医药卫生—外科学]