兔颈静脉血管内皮细胞的培养  

In Vitro Culture of Rabbit Jugular Vein Endothelial Cell

作　　者：徐岩泽[1] 胡竹林[1] 易伟斌[1] 王廷华[2] 罗哲文[1] 黄桂琴[2] 

机构地区：[1]云南省第二人民医院眼科中心,云南昆明650021 [2]昆明医学院神经科学研究所,云南昆明650021

出　　处：《昆明医学院学报》2007年第1期5-8,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助(30360108);云南省自然科学基金资助(2003C0011R);云南省教育厅科学基金资助(03Y529C)

摘　　要：目的建立稳定的兔颈静脉血管内皮细胞的体外培养方法,为自体的细胞移植提供种子细胞.方法取单侧兔颈静脉,保留对侧;用0.25%胰蛋白酶液进行灌注消化;M199培养基进行培养;Ⅷ因子相关抗原免疫组化进行血管内皮细胞鉴定.结果培养后1-3 d可见细胞贴壁,3 d后细胞变形,7-10 d细胞融合成片,14 d后可进行传代培养;Ⅷ因子相关抗原免疫组化阳性,证实所培养的细胞为血管内皮细胞;取材后实验兔一直存活,未出现死亡.结论本实验方法可以稳定地获取体外培养的兔颈静脉血管内皮细胞,并保证了实验兔的存活,为后续自体细胞移植的开展创造了条件.Objective To establish the method of culturing the jugular vein endothelial cells in vitro which may be the ideal seeding cells for cell transplantation. Methods Unilateral jugular vein obtained from an adult rabbit was dealt with 0. 25% Trypsin solution to acquire vascular endothelial cells. The vascular endothelial cells were cultured in M199 and then were identified by immunohistochemistry. Results Vascular endothelial cells adhered completely during 3 days and the morphology changed later. These cells reached confluence from 7 to 10 days, Then the vascular endothelial cells were subcultured 14 days later. The immunoreactive positive products for Factor Ⅷ related antigen were observed, which indicated the cells cultured were vascular endothelial cells. The rabbits from which we obtained unilateral jugular vein were still alive. Conclusion This method in which we obtain and culture vascular endothelial cells successfully in vitro facilitates the subsequent autogenetic cell transplantation.

关 键 词：兔 颈静脉 血管内皮 内皮细胞 培养 

分 类 号：R654.3[医药卫生—外科学]