低氧预适应对小鼠海马组织HIF-1与EPO低氧应答元件结合活性的影响  

THE EFFECT OF HYPOXIA PRECONDITIONING NO BINDING ACTIVITY OF HIF-1 ON THE HRE WITH EPO IN THE HIPPOCAMPUS OF MICE

作　　者：邵国[1] 周卫华[2] 高翠英[1] 张然[1] 吕国蔚[1] 

机构地区：[1]首都医科大学神经生物学系 [2]吉首大学医学院,湖南416000

出　　处：《中国应用生理学杂志》2007年第1期1-4,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(39670271);北京市自然科学基金资助项目(7962009)

摘　　要：目的:观察低氧预适应对小鼠海马组织HIF-1与EPO的低氧应答元件(HRE)结合活性的变化,探讨这种变化与低氧预适应形成的关系。方法:小鼠低氧0次(H0),1次(H1),4次(H4)后取海马组织,应用凝胶迁移改变试验(EMSA),染色体免疫共沉淀(ChIP)试验和荧光定量PCR(real-time PCR)技术,检测小鼠海马组织内HIF-1与EPO的低氧应答元件结合能力的变化。结果:EMSA体外结合实验及ChIP体内结合实验发现,H0、H1和H4组结合活力依次增强。结论:HIF-1与EPO的低氧应答元件结合增强可能参与预适应的形成。Aim：To observe change of binding activity of HIF-1 with erythropoietin （EPO） hypoxia response element （HRE） in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning. Methods： The hippocampus was removed from mice exposed to hypoxia for 0 run（control group）, 1 run （H1 group） and 4 runs（H4 group）. Electrophoretic mobility shift assays（EMSA）, chromatin irrmaunoprecipitation（ ChIP） and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO. Results： Both in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4. Conclusion： The increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.

关 键 词：低氧预适应 海马 HIF-1 小鼠 低氧应答元件 

分 类 号：R339[医药卫生—人体生理学]