地高辛抗血清阻断内洋地黄素对心肌缺血/再灌注损伤的影响  

EFFECT OF INHIBITING ENDOXIN BY ANTIDIGOXIN ANTISERUM ON MYOCARDIAL ISCHEMIA/REPERFUSION INJURY IN RATS

作　　者：王德国[1] 汪和贵[1] 柯永胜[1] 杨尚印[1] 

机构地区：[1]皖南医学院弋矶山医院老年心血管科,安徽芜湖241001

出　　处：《中国应用生理学杂志》2007年第1期9-12,I0001,共5页Chinese Journal of Applied Physiology

基　　金：安徽省自然科学基金资助项目(01043902);安徽省教育厅自然科学基金资助项目(99kj0219;2001kj0256)

摘　　要：目的:探讨地高辛抗血清对大鼠实验性心肌缺血/再灌注损伤(MI/R)的心肌氧化应激的影响。方法:采用左冠状动脉前降支结扎30min,复灌45min建立在体大鼠MI/R模型。SD大鼠随机分成假手术组、缺血/再灌注模型组、生理盐水组、维拉帕米组、小、中、大剂量地高辛抗血清组。于再灌注45min后检测左室心尖部缺血区心肌中内洋地黄素含量、心肌细胞膜Na+,K+-ATP酶和SOD活性以及MDA含量,光镜下观察心肌组织形态学变化。结果:MI/R组和生理盐水组大鼠心肌组织内洋地黄素水平明显升高,细胞膜Na+,K+-ATP酶活性明显下降,心肌组织SOD活性降低,MDA水平升高。治疗组包括维拉帕米组均能减轻心肌组织结构损伤,降低MDA水平,部分恢复SOD活性;但只有地高辛抗血清能降低心肌组织内洋地黄素水平,恢复心肌细胞膜Na+,K+-ATP酶活性。结论:地高辛抗血清通过拮抗内洋地黄素,恢复心肌细胞膜Na+,K+-ATP酶活性,从而减轻缺血/再灌注时的氧自由基损伤。Aim：To study the effect of antidigoxin antiserum on oxygen stress induced by myocardial ischemia/reperfusion（MI/R） injury in rats. Methods： Sprauge Dawley rats were submitted to ligate left anterior descending coronary artery 30 min followed by 45 min reperfusion. Experiment animals were randomly divided into seven groups including sham group, MI,/R group, normal salina group, verapamil group and three antidigoxin antiserum groups from low to high dose. The left ventricular myocardial tissue sample of ischemia were processed and measured the level of endoxin and malondialdehyde（MI）A）, the activities of Na^＋ ,K^＋ -ATPase and superoxin dismutase （SOD）. The myocardia morphology was odserved. Results： The levels of endoxin and MDA increased and the activities of Na^＋ ,K^＋ -ATPase and MDA were inhibited significantly in MI/R and saline groups. Including verapamil group in comparison to MI/R and saline groups, MDA level decreased and SOD activities partly reserved, meanwhile, only in three antidigoxin antiserum groups, the myocardial endoxin level was remarkably decreased, Na ^＋ , K^＋ -ATPase activities were drastically increased. The myocardial histological morphology was significantly improved. Conclusion. Antidigoxin anti,rum, an endoxin mutual clone antibody, had the effect of attenuating the damage of oxygen free radicals induced by MI/R via to antagonizing the inhibition effect of endoxin on myocardial membrane Na^＋ , K^＋ -ATPase activities

关 键 词：内洋地黄素 心肌 缺血/再灌注损伤 NA^+ K^+-ATP酶 地高辛抗血清 

分 类 号：R331[医药卫生—人体生理学] R542.2[医药卫生—基础医学]