凝闭双侧椎动脉预处理对大鼠全脑缺血损伤的防护作用  

THE PRIOR OCCLUSION OF BILATERAL VERTEBRAL ARTERIES DURING PRODUCING GLOBAL CEREBRAL ISCHEMIC DAMAGE MODEL MAY PLAY A PROTECTIVE ROLE AS PRECONDITIONING

作　　者：耿进霞[1] 张敏[1] 李文斌[1] 郭莉华[1] 李清君[1] 羡晓辉[1] 

机构地区：[1]河北医科大学基础医学院病理生理学教研室研究室,河北石家庄050017

出　　处：《中国应用生理学杂志》2007年第1期24-29,I0003,共7页Chinese Journal of Applied Physiology

基　　金：河北省自然科学基金资助项目(C200500720);教育部博士点基金项目(20050089001)

摘　　要：目的:观察凝闭双侧椎动脉与夹闭双侧颈总动脉之间的不同时间间隔对Pulsinelli四血管闭塞法全脑缺血模型的影响、以及在凝闭单侧椎动脉的基础上夹闭双侧颈总动脉后的脑缺血的特点。方法:84只Wistar大鼠,随机分为以下4组:对照组、双侧椎动脉凝闭组、全脑缺血组、单侧椎动脉凝闭+双侧颈总动脉夹闭组。全脑缺血组中,根据凝闭双侧椎动脉与夹闭双侧颈总动脉之间的时间间隔不同,又分为24 h间隔4、8 h间隔和72 h间隔3个亚组。观察大鼠脑缺血过程中的反应包括瞳孔散大、对光反射等情况,脑缺血后恢复翻正反射所需要的时间、以及动物的一般状况,并应用硫堇染色法观察海马CA1区锥体神经元迟发性死亡的情况。结果:全脑缺血72 h间隔亚组的大鼠,脑缺血过程中的反应、脑缺血后的一般状况和锥体神经元迟发性死亡程度均明显重于全脑缺血24 h间隔亚组及48 h间隔亚组,但24 h间隔亚组与48 h间隔亚组之间无显著差异。单侧椎动脉凝闭+双侧颈总动脉夹闭组大鼠的凝闭侧瞳孔散大、对光反射消失、海马CA1区神经元大量死亡;而未凝闭侧未见上述相关变化。结论:凝闭双侧椎动脉本身也具有脑缺血预处理样作用,对其后48 h内夹闭双侧颈总动脉所致的严重脑缺血具有一定程度的保护作用;大鼠椎动脉对脑干及海马的血液供应均存在明显的同侧优势效应。Aim： To study the effect of different intervals between occlusions of vertebral arteries and bilateral common carotid arteries on the Pulsinelli 4-vessel occlusion global cerebral ischemic model, and the features of ischemia of the braintem and hippocampus induced by occuluslon of bilateral common carotid arteries under the condition of occlusion of unilateral vertebral artery. Methods： Eighty four adult male Wistar rats were divided into 4 groups randomly：control group, bilateral vertebral artery occluding group, global brain ischemic insult group, and unilateral vertebral artery occluding plus bilateral common carotid arteries occluding group. In the global brain ischerrfic insult group, rats were further divided into 24 h, 48 h, and 72 h interval subgroups according to the interval between the occlusion of the vertebral arteries and bilateral common carotid arteries. The responses including enlarging of pupils and the light reflex during the brain ischemia were observed. The duration of right reflex disappearing, the general state, and the delayed neuronal death（DND） of pyramidal neurons in the CA1 hippocarnpus of the rats after the brain ischemia were also observed. Results： Among the global brain ischemic insult group, both the responses and DND were more severe in 72 h interval subgroup than those in 24 h and 48 h interval subgroups. There was no significant difference between 24 h and 48 h interval subgroups. When the bilateral common carotid arteries were occluded under the condition of occlusion of unilateral vertebral artery, severe DND was observed in the CA1 hippoeampus ipsilateral to the occluding vertebral artery, but no significant DND was observed in the contralateral CA1 hippocampus. Conclusion： The results suggested that the prior occlusion of the bilateral vertebral arteries during producing Pulsindli 4-vessel ocelusion global cerebral ischemic model might be a cerebral isehemic preconditioning that could protect to some extent pyramidal neurons of the hippocampus against severe i

关 键 词：Pulsinelli四血管闭塞法 脑缺血 脑缺血预处理 大鼠 

分 类 号：Q426[生物学—神经生物学] R338[生物学—生理学]