三种亲和层析体系纯化大豆凝集素的比较研究  

COMPARISON STUDY OF THREE AFFINITY CHROMATOGRAPHY SYSTEMS ON SOYBEAN AGGLUTININ PURIFICATION

作　　者：王利民[1] 秦贵信[1] 刘林娜[1] 张亮亮[1] 张玲玲[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118

出　　处：《大豆科学》2007年第1期51-54,共4页Soybean Science

基　　金：国家自然基金(C020302)资助项目

摘　　要：用N-乙酰基-D-半乳糖胺-epoxy-Sepharose 6B、半乳糖胺-CH-Sepharose 4B和瓜尔胶三种亲和层析体系对大豆中的凝集素进行分离提纯,并进行了多方面的比较。结果显示:三种亲和层析体系纯化大豆凝集素的产率存在显著差异,依次分别为147±15、110±12和128±16mg/100g生大豆;三种亲和胶的亲和饱和度差异显著,分别为12.2±1.4、7.5±1.3和2.1±1.6 mg/mL凝胶。这一结果表明大豆凝集素最理想的纯化方法是N-乙酰基-D-半乳糖胺-expoxy-sepharose 6B亲和层析体系。Purification of soybean agglutinin with three affinity chromatography systems, GalNac -- epoxy-- Sepharose 6B, Gal-- CH-- Sepharose 4B and guar were compared. The results showed the differences of the prodution rate and absorbing capcities among the three systems were significant, 147±15,110±12, 128±16mg/100g raw soybean and 12.2±1.4,7.5±1.3, 2.1±1.6 mg/ mL gel separately. This result indicated that the best way to purify soybean agglutinin was Gal- Nac- epoxy-Sepharose 6B.

关 键 词：亲和层析 纯化 大豆凝集素 

分 类 号：S565.101[农业科学—作物学]