Resistin-binding peptide antagonizes role of resistin on white adipose tissue  

作　　者：Nan GU Shu-ping HAN Li FEI Xiao-qin PAN Mei GUO Rong-hua CHEN Xi-rong GUO 

机构地区：[1]Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210029, China [2]Pediatric Research Institute of Nanjing Medical University, Nanjing 210029, China

出　　处：《Acta Pharmacologica Sinica》2007年第2期221-226,共6页中国药理学报（英文版）

基　　金：grants from the National Natural Science Foundation of China(No 30371502);the Natural Science Foundation of Jiangsu Province(No BK2001120);a grant from Jiangsu Province Health Department(No RC2002061).

摘　　要：Aim： To investigate the direct effects of resistin and resistin-binding peptide （RBP） on lipid metabolism and endocrine function in adipose tissue. Methods： Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein （rResistin） or combined with RBP of varying concentrations （ 1 × 10^12 mol/L, 1 × 10^-10 mol/L, 1 × 10^-8 mol/L）. Free fatty acids （FFA） released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-α and adiponectin were detected by ELISA kit and RT-PCR respectively. Results： The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-α in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1×10^-8 mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations. Conclusion： RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.Aim： To investigate the direct effects of resistin and resistin-binding peptide （RBP） on lipid metabolism and endocrine function in adipose tissue. Methods： Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein （rResistin） or combined with RBP of varying concentrations （ 1 × 10^12 mol/L, 1 × 10^-10 mol/L, 1 × 10^-8 mol/L）. Free fatty acids （FFA） released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-α and adiponectin were detected by ELISA kit and RT-PCR respectively. Results： The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-α in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1×10^-8 mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations. Conclusion： RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.

关 键 词：RESISTIN resistin-binding peptide white adipose tissue lipid metabolism endocrine function 

分 类 号：Q591.5[生物学—生物化学] Q593.5