家族性进行性色素过度沉着症一家系B淋巴细胞系建立及意义  

Establishment and Its Significance of B-lymphocyte Cell Lines in A Chinese Family with Familial Progressive Hyperpigmentation

作　　者：邓云华[1] 张彩娥[2] 陈兴平[1] 吴雄文[2] 周礼义[1] 陈映玲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院皮肤科,湖北武汉430030 [2]华中科技大学同济医学院免疫学系,湖北武汉430030

出　　处：《中国皮肤性病学杂志》2007年第2期69-71,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的为保证对一个汉族家族性进行性色素过度沉着症(FPH)家系致病基因研究的顺利实施,对该家系成员进行B淋巴细胞转化并建立永生化细胞系。方法采用EBV转化外周血B淋巴细胞并加用环孢霉素A。结果成功建立该家系成员永生化细胞系15个;B淋巴母细胞系(B-LCL)成功转化后其体积增大,形态多样,伸出伪足,增生的淋巴细胞多聚集成团。结论该家系成员B-LCL建系成功,为后续定位克隆FPH基因并进行皮肤色素代谢障碍的分子机制研究提供了长期的研究材料。Objective To investigate the further pathomechanism on familial progressive hyperpigmentation （FPH） in a Chinese pedigree with 17 members in Hubei province,permanent B lymphocyte cell lines （B-LCL） of this family were established. Methods The immortal B-LCLs of the family were established by EBV transformation and addition of cyclosporin A（CysA） to inhibit the activity of T cells. Results 15 immortal, cell lines were established. The results showed that cellular volume of these immortal cells were enlarged and their morphology was diversity with glitch. There was increasing development of cell aggregates of proliferative lymphocyte cells. Conclusion The immortal B-LCLs acquired successfully provide a long and plentiful DNA material for mapping and cloning causative gene for FPH and studying punctually the molecular mechanism of pigment metabolism in the skin.

关 键 词：家族性进行性色素过度沉着症 B淋巴细胞系 

分 类 号：R758.5[医药卫生—皮肤病学与性病学]