体外培养海马神经元糖氧剥夺损伤模型的建立及川芎嗪对其胞内钙离子的影响  

Establishment of Oxygen and Glucose Deprive Model of in vitro Cultured Hippocampal Neuron and Effect of Ligustrazine on Intracellular Ca^(2+) Level in Model Neurons

作　　者：万海同[1] 王玉[1] 杨洁红 朱慧渊[1] 郭莹[1] 严伟民[2] 韩进[1] 

机构地区：[1]浙江中医药大学心脑血管病研究所,杭州310053 [2]浙江大学生物医学与仪器科学学院

出　　处：《中国中西医结合杂志》2007年第3期234-236,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(No.30371789;30672652);浙江省自然科学基金项目(No.Z204424)

摘　　要：目的研究川芎嗪对体外培养海马神经元糖氧剥夺损伤细胞内钙离子[Ca^(2+)]i的影响。方法建立体外培养海马神经元糖氧剥夺(OGD)模型,应用激光共聚焦显微技术检测不同剂量川芎嗪对OGD状态下海马神经元内Ca^(2+)的影响。结果成功建立海马神经元OGD模型,经检测模型组[Ca^(2+)]i高于空白对照组(P＜0．05);阳性药组和川芎嗪大、中剂量组[Ca^(2+)]i均低于模型组(P＜0．05)。结论海马神经元OGD存在钙超载,川芎嗪具有钙拮抗作用,对脑组织有保护作用。Objective To establish the oxygen and glucose deprive （OGD） model in cultured hippocampal neuron and study the effect of ligustrazine on intracellular Ca^2 ＋ level in the model neurons. Methods The OGD model was established in cultured hippocampal neuron, and the intracellular Ca^2 ＋ level in it was detected by laser scanning confocal microscope （LSCM）. Results The OGD model was successfully established in cultured hippocampal neurons; the intracellular Ca^2 ＋ level in the OGD model group was significantly higher than that in the blank control group （P 〈 0.05）, and that in the nemodipine and high and medium dosage of ligustrazine treated groups was lower than that in the OGD model group （P 〈 0.05）. Conclusion Intracellular Ca^2 ＋ overload occurs in OGD model neuron, which could be antagonized by ligustrazine, indicating that ligustrazine has a protective effect on hippocampal neuron from hypoxic-ischemic injury.

关 键 词：川芎嗪 海马神经元 细胞培养 脑缺血 缺氧 钙离子 

分 类 号：R285.5[医药卫生—中药学]