银杏叶提取物金纳多心停跳液对人心肌细胞bcl-2和bcl-xL基因表达的影响  

Effects of Ginkgo biloba Extract (Ginaton) on mRNA Expression of bcl-2 and bcl-xL in Myocardium of Patients Underwent Hypothermic Cardiopulmonary Bypass

作　　者：邓云坤[1] 韦方[1] 李永念[2] 陈静[1] 刚绍鹏[1] 

机构地区：[1]贵州省人民医院,贵阳550002 [2]贵阳医学院免疫教研室

出　　处：《中国中西医结合杂志》2007年第3期256-258,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：贵州省优秀科技教育人才省长专项资金项目(No.黔省专合字[2005]34);贵州省中医药管理局资助项目(No.2004-1)

摘　　要：目的探讨银杏叶提取物金纳多(简称金纳多)对体外循环人心肌细胞抗凋亡基因bcl-2和bcl- xL表达的影响。方法择期先天性心脏病患儿30例,随机均分为两组,治疗组在灌注St Thomas'停搏液(4℃)中加入金纳多(0．5 mg/kg),心脏完全停跳后开始心内手术;对照组单纯灌注St Thomas'停搏液(4℃)。在手术结束开放升主动脉前取心肌组织,采用RT-PCR技术在凝胶电泳成像基础上半定量检测bcl- 2和bcl-xL基因的表达水平。结果治疗组bcl-2和bcl-xL基因的表达显著高于对照组(P＜0．05)。结论银杏叶提取物金纳多可促进人心肌细胞抗凋亡基因bcl-2和bcl-xL的表达。Objective To investigate mRNA expression in the myocardium of Methods Thirty congenital heart disease the effects of Ginkgo biloba extract （Ginaton） on bcl-2 and bcl-xL patients underwent hypothermic cardiopulmonary bypass （CPB）. patients were randomly assigned to 2 groups, the control group and the treated group. Patients in both groups received St. Thomas＇ cardioplegic solusion via radix aortae, while Ginaton （0.5mg/kg） was added in the treated group. Cardiac surgery was started after complete heart arrest. Myocardium was taken before the aorta ascendens was unblocked and mRNA expression of bcl-2 and bcl-xL in the ventricular tissue was detected by RT-PCR. Results The gene expressions of bcl-2 and bcl-xL were significantly higher in the treated group than those in the control group （P 〈 0.05）. Conclusion Ginaton could promote the mRNA expressions of the antiapoptotic gene bcl-2 and bcl-xL in myocardium of patients underwent CPB.

关 键 词：银杏叶提取物(金纳多) BCL-2 bcl—xL 基因表达 

分 类 号：R285.5[医药卫生—中药学]