23-羟基白桦酸诱导LoVo细胞凋亡与线粒体膜电位的变化  

Apoptotic activity of 23-Hydroxybetulinic acid and the change of mitochondrial membrane potential in human colorectal cancer cell line LoVo

作　　者：程燕[1] 叶文才[1] 石俊敏[1] 姚新生[1] 栗原博[1] 

机构地区：[1]暨南大学中药及天然药物研究所,广东广州510632

出　　处：《中国药理学通报》2007年第3期350-353,共4页Chinese Pharmacological Bulletin

基　　金：广东省关键领域重点突破资助项目(No2003A30909);广州市重点科技攻关资助项目(No2004Z2-E5021)

摘　　要：目的探讨23-羟基白桦酸对人结肠癌细胞株LoVo细胞增殖和凋亡的影响。方法采用MTT法检测23-羟基白桦酸对LoVo细胞的增殖抑制作用。Hoechst染色、流式细胞仪检测细胞凋亡及线粒体膜电位的变化。结果23-羟基白桦酸能抑制LoVo细胞的增殖,其作用呈明显的时间和剂量依赖性。Hoechst33258染色显示细胞凋亡特征。23-羟基白桦酸25、50、100及200μmol·L-1作用LoVo细胞48h后,其凋亡率分别为11.32%±0.92%、19.23%±0.78%、24.51%±5.88%及42.22%±2.32%,显示一定的剂量依赖性关系。与空白组相比,23-羟基白桦酸可明显降低LoVo细胞线粒体膜电位(P<0.01)。结论23-羟基白桦酸可抑制LoVo细胞的增殖,其机制与降低线粒体膜电位继而诱导LoVo细胞凋亡有关。Aim To investigate the apoptotic effects of 23-hydroxybetulinic acid in human colorectal cell line LoVo and the mechanism of apoptosis. Methods MTT-based cytotoxicity assay was used to test the LoVo cell proliferation. The apoptotic cells and the mitochondrial membrane potential were detected by fluorescent miroscopy, flow cytometric analysis. Results 23-Hydroxybetulinic acid inhibited LoVo cell proliferation in a dose and time-dependent manner. Hochst33258 images showed that most cells exposed to 23-hydroxybetulinic acid exhibit apoptotic morphology change. By FCM methods, the apoptotic rates of LoVo cells were 11.32% ±0.92%, 19.23% ±0.78%,24. 51% ± 5.88%, 42. 22% ± 2.32%, after treatment of 23-hydroxybetulinic acid at concentrations 25, 50, 100,200 μmol · L^-1, which showed obvious concentration-dependent relationship. Compared with control group, 23-hydroxybetulinic acid caused a concomitant dissipation of the mitochondrial membrane potential, which resulted in LoVo cell apoptosis. Conclusion 23-Hydroxybetulinic acid could inhibit LoVo cell proliferation. The dissipation of mitochondrial membrane potential may result in the LoVo cell apoptosis.

关 键 词：23-羟基白桦酸 LOVO细胞 细胞凋亡 线粒体膜电位 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]