中草药对柔嫩艾美耳球虫卵囊孢子化的影响  

Study on Sporulating of Eimeria tenella Oocyts Controlled by Chinese Medicinal Herbs

作　　者：葛凯[1] 李培英[1] 汪兴生[2] 曾明华[1] 李生龙 徐前明[1] 顾有方[4] 吴金节[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽中医学院药学院,安徽合肥230038 [3]安徽华澳生物技术有限公司,安徽合肥231131 [4]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2007年第1期1-5,共5页Journal of Anhui Science and Technology University

基　　金：安徽省教育厅自然科学重点项目(2005KJ025ZD)

摘　　要：为筛选有效的体外抗球虫中草药,研究了苦参、商陆等23味中草药对柔嫩艾美耳球虫卵囊孢子化的影响。将未孢子化球虫卵囊分别在质量百分浓度为25%、50%、100%的上述药物中进行培养,分别在第18h,24h,36h,48h观察球虫卵囊的孢子化率。然后洗去药物,在重铬酸钾中继续培养,分别在第18h,24h,36h观察球虫卵囊的孢子化率。结果表明:在23味中草药中,苦参等抑制效果不明显;商陆等具有较好的抑制作用,随时间的延长,球虫卵囊的孢子化率呈下降趋势,且药物浓度越高,抑制效果越好。To selecting effective anti - coccidian Chinese medicinal herbs, 23 kinds of Chinese medicinal herbs, such as Radix Sophorae Flavescentis ,Radix Phytolaccae,were Chose to control the sporulating of Eimeria teneUa oocyts. The unsporulated oocyts were cultivated in those herbs. Every herbs were made into three concentration -25 percent,50 percent,100 percent. The rate of sporulate was observed at 18h ,24h ,36h ,48h. Then the drugs were centrifugaled and separated, the oocyts were cultivated in K2Cr207 solution. The rate of sporulate was observed at 18h ,24h ,36h. The results indicated that the controlling function of some drugs were feeble, for exampie Radix Sophorae Flavescentis etc, others were good, for example Radix Phytolaccae etc. And when the time extended, the rate of sporulate descended gradually. Moreover, the higher the concentration of drugs, the better the controlling function of drugs.

关 键 词：中草药 柔嫩艾美耳球虫卵囊 孢子化 抑制作用 

分 类 号：S853.7[农业科学—临床兽医学]