组织型纤溶酶原激活因子对生物材料表面血小板黏附的影响  

Effect of tissue-typed plasminogen activator on the adhesion of platelets on the surface of biomaterials

作　　者：林慧庆[1] 舒胜强[2] 黄杰[2] 

机构地区：[1]华中科技大学同济医学院协和医院胸外科,湖北省武汉市430030 [2]武汉大学人民医院心血管内科,湖北省武汉市430060

出　　处：《中国组织工程研究与临床康复》2007年第9期1651-1653,1658,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察组织型纤溶酶原激活因子包被涤纶和膨体聚四氟乙烯材料对表面血小板黏附影响的体内和体外实验。方法:实验于2004-06/2005-06在武汉大学人民医院心内科实验室(国家211重点实验室)完成。选择雄性新西兰大耳白兔72只,随机数字表法分为3组:空白对照组、谷胱苷肽硫基转移酶蛋白组和组织型纤溶酶原激活因子重组蛋白组,每组24只。每组又分涤纶材料和膨体聚四氟乙烯材料。实验用材料:涤纶B型(苏州华明织带有限公司),膨体聚四氟乙烯(美国Gore&Associates公司)。谷胱苷肽硫基转移酶和谷胱苷肽硫基转移酶-组织型纤溶酶原激活因子融合蛋白由实验室制备。手术分离兔股动脉,夹闭股动脉两端,间隔>6cm,用眼科剪剪开0.3cm左右的纵口,用生理盐水将血管内的血液冲洗干净,然后放入经谷胱苷肽硫基转移酶蛋白(谷胱苷肽硫基转移酶蛋白组)或谷胱苷肽硫基转移酶-组织型纤溶酶原激活因子蛋白(组织型纤溶酶原激活因子重组蛋白组)包被处理的两种材料膜片。空白对照组进行手术放入未经处理的两种材料膜片后血管缝合,不予以任何处理。通过体内和体外动物模型实验,观察血小板在材料表面的黏附情况和对机体内血小板黏附的影响。结果:纳入大耳白兔72只,均进入结果分析。①体外实验结果显示,在涤纶材料的实验中,组织型纤溶酶原激活因子重组蛋白组和谷胱苷肽硫基转移酶蛋白组血小板在材料表面黏附数目低于空白对照组,减少了大约76.4%,42.3%(分别为4.3×109L-1,10.5×109L-1,18.2×109L-1,χ2=20.15,P<0.01)。在聚氯乙烯材料的实验中,也得到了类似的结果,组织型纤溶酶原激活因子重组蛋白组和谷胱苷肽硫基转移酶蛋白组血小板在材料表面黏附数目低于空白对照组,减少了大约74.8%,34.1%(分别为3.1×109L-1,8.1×109L-1,12.3×109L-1,χ2=22.65,P<0.01,0.05)。②在体实验结果表明,在两�AIM： To research the adhesion of platelets on Dacron and polytetrafluoroethylene （PTFE） coated by tissue-typed plasminogen activator （t-PA） materials through experiments in vivo and vitro. METHODS： The experiment was accomplished in the Laboratoey of Cardiology, the People＇s Hospital of Wuhan University （State Key Laboratory of the 211 Project） between June 2004 and June 2005. A total of 72 healthy male New Zealand rabbits were randomly divided into three groups： control group, glutathione S-transferase （GST） protein group and GST-t-PA recombinant protein group. Each group of 24 rabbits was randomly assigned into Dacron matertal group and PTFE material group. Experimental materials： Dacron B （Suzhou Minghua Weave Ribbon Co.,Ltd）, PTFE （Gore＆Associates Company）. GST and GST-t-PA fusion protein were prepared by this laboratory. Femoral artery were dissected, clamped and incised with 0.3 cm area by eye scissors, with the interval of more than 6 cm. After washing by saline, the incision was put with material chaff coated with GST protein or GST-t-PA fusion protein in the artery of corresponding groups. In the control group, the incision was sutured without materials. The adhesion of platelets on Dacron and PTFE coated by t-PA materials was observed through experiment in vivo and vitro. RESULTS： All 72 rabbits were involved in the result analysis.（1）In vitro experiment： on Dacron materials, the adhesion of platelets in GST group and GST-t-PA group were lower than those of control groups, decreasing 76.4% and 42.3% （4.3×10^9 L^-1, 10.5×10^9 L^-1, 18.2×10^9 L^-1, respectively, Χ^2 =20.15, P 〈 0.01）. As for PTFE materials, the two groups showed the identical trend and decreased 74.8% and 34.1% compared with control group （3.1×10^9 L ^-1, 8.1×10^9 L^-1, 12.3×10^9 L^-1, Χ^2= 22.65, P〈 0.01, 0.05）. （2）In vivo experiment： on both materials, the adhesion of platelets in GST-t-PA group were obviously lower than those of GST group and control group （Dacr

关 键 词：组织型纤溶酶原激活因子 谷胱苷肽硫基转移酶 血小板黏附 

分 类 号：R318.1[医药卫生—生物医学工程]