蔗蜡高级烷酸和高级烷醇分离与降胆固醇的作用(英文)  

Separation of high-class alkanols and high-class eicosanoic acids in sugar cane wax and activity of reducing blood cholesterol

作　　者：刘方欣[1] 张玉诺[1] 李晓愚[1] 谢娟[1] 王敏 刘先俊[1] 

机构地区：[1]重庆医科大学放射医学教研室,重庆市400016 [2]重庆康尔威药业股份有限公司,重庆市401122

出　　处：《中国组织工程研究与临床康复》2007年第9期1772-1775,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:甘蔗蜡作为制糖工业的副产品,在非医药方面已有广泛的用途。有实验证实,甘蔗蜡在降低血胆固醇方面具有良好的作用,但其效应及适用于医药应用的甘蔗蜡的分离手段有待进一步研究。目的:从国产甘蔗蜡中分离适合医药用途的高级烷酸混合物、高级烷醇混合物,并观察其对高脂血症模型大鼠的降胆固醇作用。设计:随机对照动物实验。单位:重庆康尔威药业股份有限公司药物研究所。材料:实验于2005-04/2006-01在重庆康尔威药业股份有限公司药物研究所完成。选用65只成年Wistar雌性大白鼠,体质量180～220 g,鼠龄3～6个月,SPF级,由重庆中药研究院实验动物中心提供。粗甘蔗蜡购自北京嘉德鸿盛生物化学科技有限公司。26C醇、28C醇、30C醇、32C醇和28C酸、30C酸、32C酸、34C酸为Sigma产品(气相色谱标准品)。其余试剂为国产分析纯级。方法:①将粗甘蔗蜡通过乙醇等有机溶剂反复提取后成为精制甘蔗蜡,进一步通过皂化、加钙等,将高级烷酸混合物与高级烷醇混合物分离。用气相色谱分析其主要成分,高级烷醇混合物主要分析C26、C28、C30和C32,高级烷酸混合物主要分析C26、C28、C30和C32。②按照参考文献叙述的方法,将大白鼠适应性饲养3d后,随机摸球法分为空白组(n=10)和实验组(n=55),断尾采血,自动生化分析仪测定所有大鼠三酰甘油、总胆固醇与高密度脂蛋白胆固醇。空白组给予普通颗粒饲料,实验组给予高脂饲料(猪油质量分数0.1、蛋黄粉质量分数0.1、胆固醇质量分数0.01、猪胆盐质量分数0.002、普通饲料质量分数0.788)[3],自由摄食与饮水。7 d后尾尖再次采血测定三酰甘油、总胆固醇与高密度脂蛋白胆固醇。按血清三酰甘油的高低随机将实验组大白鼠分为5个亚组:阴性对照组、高级烷醇低剂量组、高级烷醇高剂量组、高级烷酸低剂量组、高级烷酸高剂量组,每组1BACKGROUND：As a by-product in sugar industry, sugar cane wax has been widely used in non-medical field. Some researches indicate that sugar cane wax plays a great role in reducing blood cholesterol; however, the therapeutical effect and clinical application should be studied further. OBJECTIVE ： To separate the high-class eicosanoic acid and the high-class alkanols, which are suitable for medical application, and further to observe the effect of them on reducing blood cholesterol of model rats with hyperlipemia. DESIGN： Randomized control animal study SETTING： Pharmacological Institute, Chongqing Kangerwei Pharmaceutical Co., Ltd MATERIALS： The experiment was carried out in the Pharmacological Institute of Chongqing K.E.W Pharmaceutical Co., Ltd. from April 2005 to January 2006. A total of 65 adult female Wistar rats, aged at 3-6 months, weighing 180-220 g, of SPF grade, were provided by Experimental Animal Center of Chongqing Institute of Traditional Chinese Medicine. Raw sugar cane wax was provided by Beijing Jiade Hongsheng Biochemical Technology Co., Ltd. high-class alkanols C26, C28, C30, C32 and high-class eicosanoic acid C26, C26, C30, C32 were provided by Sigma Company （standard materials of gas phase chromatography）, and other reagents were national analytical pure. METHODS ： （1） Sugar cane wax was extracted from raw sugar cane wax with ethanol and other organic solution and separated from the mixture of high-class eicosanoic acid and the mixture of high-class alkanols with saponification and calcification. Main components were analyzed with gas phase chromatography. The main components of high-class alkanols were C26, C28, C30 and C32 and the main components of high-class eicosanoic acid were C28, C30, C32 and C34.（2） Based on references, rats were fed in 3 days and randomly divided into blank group （n =10） and experimental group （n =55）. And then, all rats were cut off their tails to collect blood and the triacylglycerol （TG）, total cholesterol （TC） and

关 键 词：甘蔗蜡 高级烷醇混合物 高级烷酸混合物 胆固醇 

分 类 号：R318.08[医药卫生—生物医学工程]