Combination of hollow fiber-based liquid-phase microextraction with sweeping techniques in micellar electrokinetic chromatography for the determination of Strychnos alkaloids in human urine  

作　　者：Xiao Huan Zang Cai Rui Li Qiu Hua Wu Chun Wang Dan Dan Han Zhi Wang 

机构地区：[1]Key Laboratory of Bioinorganic Chemistry, College of Science, Agricultural University of Hebei, Baoding 071001, China [2]College of Vocational and Technological Sanitation, Hebei University, Baoding 071002, China

出　　处：《Chinese Chemical Letters》2007年第3期316-318,共3页中国化学快报（英文版）

基　　金：This work was supported both by the Natural Science Foundation of Hebei Province(B2006000413);the Scientific Research Foundation for the Returned 0verseas Chinese Scholars,State Education Ministry.

摘　　要：A new method for the enrichment of Strychnos alkaloids in biological samples via liquid-phase microextxaodon （LPME） based on porous polypropylene hollow fibers in combination with on-line sweeping in micellar electrokinodc chromatography was developed. The calibration curve was linear over the range of 20-200 ng mL^-1 for both strychnine and brucine in human urine sample. The detection limits （S/N = 3：1） for strychnine and brucine were 1 ng mL^-1 and 2 ng mL^-1, respectively. The LPME-sweeping method has been successfully applied to the analysis of strychnine and brucine in real urine samples.A new method for the enrichment of Strychnos alkaloids in biological samples via liquid-phase microextxaodon （LPME） based on porous polypropylene hollow fibers in combination with on-line sweeping in micellar electrokinodc chromatography was developed. The calibration curve was linear over the range of 20-200 ng mL^-1 for both strychnine and brucine in human urine sample. The detection limits （S/N = 3：1） for strychnine and brucine were 1 ng mL^-1 and 2 ng mL^-1, respectively. The LPME-sweeping method has been successfully applied to the analysis of strychnine and brucine in real urine samples.

关 键 词：Hollow fiber-based liquid-phase microextraction SWEEPING MEKC Strychnos alkaloids URINE 

分 类 号：R446.129[医药卫生—诊断学]