当归多糖硫酸酯的抗氧化作用研究  

Anti-oxidative Effect of Angelica Polysaccharide Sulphate

作　　者：贾敏[1] 杨铁虹[1] 姚秀娟[1] 孟嘉[1] 孟静茹[1] 梅其炳[1] 

机构地区：[1]第四军医大学药理学教研室,陕西西安710032

出　　处：《中药材》2007年第2期185-188,共4页Journal of Chinese Medicinal Materials

基　　金：陕西省中医管理局资助项目(0347)

摘　　要：目的：观察当归多糖硫酸酯（APS）的抗氧化损伤作用。方法：常规方法体外培养Hela细胞，加入不同浓度的APS孵育24h后，用H2O2或紫外线照射诱导细胞氧化损伤。APS抗氧化损伤作用检测如下：MTT法测定细胞活力；比色法测定胞浆内SOD活力（黄嘌呤氧化酶法）、GSH和MDA。结果：H2O2或紫外线照射损伤的Hela细胞活性明显下降，胞浆中的GSH、SOD活性显著降低，MDA含量升高，与对照组比有显著性差异（P〈0．05或P〈0．01），表现出明显的氧化损伤特点。APS在0．3—100μg／ml浓度范围内，剂量依赖性地增加细胞活力，升高GSH、SOD的活性，降低MDA的含量，与模型组比有显著性差异（P〈0．05或P〈0．01）。结论：APS具有抗细胞氧化损伤的作用，这可能是其抗AIDS作用的机理之一。Objective ： To investigate the anti-oxidative effect of Angelica polysaccharide sulphate（APS）. Methods ： The Hela cells were cultured conventionally. Then APS was added and cultured together in different concentration for 24h followed by a oxidative injury with H2O2 or UV irradiation. The anti-oxidative effects of APS were detected as follow：cell viability was measured by MTT assay;colorimetric analysis was used to determine SOD activity, GSH and MDA level in cytoplasm. Results： Treatment of H2O2 or UV irradiation significantly decreased cell viability, GSH and SOD activity in cytoplasm, while increased MDA in cytoplasm. At the range of 0. 3 - 100μg/ml,APS significantly increased cell viabilty, GSH and SOD activity, while decreased MDA in a dose dependent manner（ P 〈 0. 05 or P 〈 0.01 ）. Conclusion： APS has anti-oxidative effect, which may be one of its anti-AIDS mechanisms.

关 键 词：当归多糖 多糖硫酸酯 氧化损伤 

分 类 号：R285.5[医药卫生—中药学]