和血益智方对VD大鼠学习记忆和海马的影响  

Effects of Hexue Yizhi Formula on Learning and Memory and Morphology of Hippocampal Neurons of Rat with Vascular Dementia

作　　者：田先翔[1] 梅琼彬[1] 王立[2] 袁芳[2] 袁尚荣[2] 吴正治 

机构地区：[1]湖北中医学院药理学教研室,湖北武汉430065 [2]湖北中医学院解剖组胚教研室,湖北武汉430065 [3]深圳市中西医结合临床研究所

出　　处：《深圳中西医结合杂志》2007年第1期6-9,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：湖北省教育厅资助课题(2004X082);湖北中医学院科研课题

摘　　要：目的观察和血益智方对VD大鼠行为学和形态学的影响。方法动物随机分为假手术组和模型组。VD模型制作成功后随机分为和血益智方小剂量组和大剂量组、思尔明组、生理盐水(NS)组。用穿梭箱和水迷宫检测大鼠行为学并作海马HE常规染色,光镜观察。结果和血益智方、思尔明均能缩短VD大鼠穿梭箱实验主动逃避时间和遭受电击时间,减少遭受电击次数;能缩短VD大鼠水迷宫实验时定位航行潜伏期、游泳的路径,增加大鼠空间探索实验中穿越原目标的次数。NS组海马细胞体积变大、核变小、有核仁消失和核固缩;除假手术组正常外,其余各组海马细胞层次有减少,但细胞排列比较密集、细胞形态尚可。结论和血益智方可改善VD大鼠的学习记忆能力。Objective To study the effects of Hexue Yizhi Formula on learning and memory and the morphology of the hippocampal neurons of the rats with Vascular Dementia （VD）. Methods The rats were subjected for ischemia-reperfusion repeatedly by blocking the bilateral common carotid arteries to establish VD models. They were divided into four groups randomly after the establishment of VD model. The treating groups were administrated with Hexue Yizhi Formula （1 mL/100 g）, Hexue Yizhi Formula （2 mL/100 g）, Sermion, Natural Salt Solution （NS） respectively. Animals with the shamed-operation were taken as control. The behavior changes were observed through the Shuttle box test and water maze test at the end of treatment. Results In shuttle box test Stimulus Frequency （SC）, Stimulated Time （ST） and Active avoidance Response Time （ART） of the rats were reduced respectively in Sermion, Hexue Yizhi Formula （1 mL/100 g）, Hexue Yizhi Formula （2 mL/100 g） groups than those in NS group. In the Morris water maze test, Stage of Latency and Pathway were declined and the times were increased markedly acrossing the target. The structure levels of hippocampal neurons reduced, but cells were more normal and intensive in treated groups except NS group. Conclusion Our study suggest that Hexue Yizhi Formula can improve the ability of the leaning and memory of the rats with vacular dementia and improve the clinical symptoms of VD.

关 键 词：血管性痴呆 MORRIS水迷宫 学习记忆 海马 

分 类 号：R-332[医药卫生]