XRCC1、XPD、XRCC3、CCND1基因多态性与职业慢性苯中毒发病工龄的关联  

Analysis for the association between genetic polymorphisms of XRCC1,XPD,XRCC3,CCND1 and the latency of the occupational chronic benzene poisoning

作　　者：许建宁[1] 黄慧隆[2] 王全凯[1] 王雅文[1] 杨敏[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所毒理室,北京100050 [2]铁道科学研究院环控劳卫研究所

出　　处：《中华预防医学杂志》2007年第2期114-117,共4页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金(30300279)

摘　　要：目的探讨 DNA 损伤修复基因 XRCC1、XPD、XRCC3基因和细胞周期调控基因CCND1的多态性与慢性苯中毒发病工龄的关系。方法以确诊的80名慢性苯中毒患者作为研究对象,应用 PCR-RFLP 分析技术,检测 DNA 损伤修复基因 XRCC1(C26304T、G27466A、G28152A、G36189A 位点)、XPD(C22541A、C23591T、A35931C 位点)、XRCC3(C18067T 位点)和细胞周期调控CCND1基因 G870A 位点的多态性,比较不同基因型 Kaplan-Meier 生存曲线,评价慢性苯中毒发病工龄与 XRCC1、XPD、XRCC3和 CCND1基因多态性的关联。结果携带 XRCC1 27466G/G 基因型的个体发生慢性苯中毒的发病工龄比携带27466G/A+A/A 变异基因型的个体长6.9年;携带 CCND1870G/G 基因型的个体比 G/A+A/A 基因型个体的发病工龄短6.2年。结论 XRCC1和 CCND1基因多态性可能影响慢性职业性苯中毒的发病工龄。Objective To explore the association between genetic polymorphisms of XRCC1, XPD, XRCC3 and CCND1 and latency of occupational chronic benzene poisoning. Methods 80 patients diagnosed with occupational chronic benzene poisoning were investigated. PCR-RFLP was applied to detect the single nucleotide polymorphisms of C26304T, G27466A, G28152A, G36189A of XRCC1, C22541A, C23591T, A35931C of XPD, C18067T of XRCC3 and G870A of CCND1. Their relationship with the latency of chronic benzene poisoning was analyzed by Kaplan-Meier method. Results The association of XRCC1 G27466A subgroup with the latency of chronic benzene poisoning was observed, as well as that of CCDN1G870A subgroup. The benzene-exposed workers with XRCC1 27466G/G homozygous wild genotype developed chronic benzene poisoning 6. 9 years later than those had homozygous （27466A/A） or heterozygous （27466G/A） mutant alleles. On the other hand, the latency developing chronic benzene poisoning was longer in workers with homozygous （CCNDI 870A/A） or heterozygous （CCNDI 870G/A） mutant alleles than in those carrying 870G/G homozygous wild genotype （14. 9 vs 8.7 years ）. Conclusion The polymorphisms of XRCC1 and CCND1 potentially modify the latency of the chronic benzene poisoning among workers exposed to benzene.

关 键 词：DNA修复 基因多态性 慢性苯中毒 发病工龄 

分 类 号：R135.1[医药卫生—劳动卫生]