前列腺素A1对兔供肺缺血再灌注损伤的保护作用  

Protective effects of prostaglandin A1 on ischemia-reperfusion injury during rabbit lung-transplant

作　　者：朱红军[1] 于曙东[1] 何靖康[1] 韩蓉[1] 邬郡超[1] 杨美菊[1] 刘德森[1] 

机构地区：[1]苏州大学附属第一医院心血管外科,215006

出　　处：《中华实验外科杂志》2007年第3期277-278,F0003,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省高校自然科学基金资助项目（02122527）

摘　　要：目的探讨前列腺素A1在兔肺移植中对供肺缺血再灌注肺保护作用及其作用机制。方法将16对健康家兔随机分为两组，对照组肺以改良低钾右旋糖酐（LPD）肺保护液灌注及保存，实验组则将前列腺素A1（80ng／L）加入改良LPD液灌注及保存，在4℃保存4h后再灌注1h，测定移植肺组织的干湿比（W／D）；测定肺组织中髓过氧化物酶（MPO）活性、核转录因子-kB（NF-kB）的表达以及细胞间黏附分子-1（ICAM-1）的表达；并观察肺组织病理结构的变化。结果再灌注后，实验组的W／D和MPO也明显低于对照组（P〈0．01），其肺组织中NF-kB和ICAM-1的表达也明显低于对照组（P〈0．01），肺组织病理变化较对照组轻。结论前列腺素A1能减轻肺的缺血再灌注损伤，有效改善肺功能，对供肺具有明显的保护作用。Objective To investigate the protective effects of prostaglandin A1 on ischemiareperfusion injury during rabbit lung-transplam, and explore its possible mechanism. Methods Sixteen pairs of rabbits were randomly divided into two groups：the control group and the experimental group. The harvested lung blocks were flushed with and stored in the modified low-postasssium-dextron （LPD） solution in control group, but in the experimental group LPD containing prostaglandin A1 （80 ng/L） was used. The lung was stored at 4 ℃for 4 h in both groups. The rabbit lung-transplant reperfusion models were established and the lungs were perfused for 1 h. After reperfusion,the lung tissue wet-to-dry （W/D） ratio and myeloperoxidase （MPO） activity were measured. The protein expression of nuclear factor-kappa B （NF-kB） and intercellular adhesion molecule-1 （ICAM-1） was also detected at the end of reperfusion. Light microscopic and ultrastructural changes were also examined. Results After reperfusion the W/D, MPO,and the protein expression of NF-kB and ICAM-1 were decreased evidently in the experimental group （ P 〈 0.01 ）. The morphologic and ultrastructual damages in the experimental group were milder than in the control group. Conclusion Prostaglandin A1 can alleviate the ischemia-reperfusion injury of lung and improve the protective effect of lung preservation.

关 键 词：肺保护 缺血 再灌注损伤 前列腺素 核因子-KB 

分 类 号：R686[医药卫生—骨科学]