大鼠肺微血管内皮细胞的培养  

Culture of Pulmonary Microvascular Endothelial Cells in Rats

作　　者：陈瑞华[1] 赵自刚[1] 牛春雨[1] 张静[1] 张玉平[1] 

机构地区：[1]河北北方学院病理生理教研室,河北张家口075029

出　　处：《中国微循环》2007年第1期16-19,共4页Journal of Chinese Microcirculation

基　　金：国家自然科学基金资助项目(No.30370561);河北省自然科学基金资助项目(No.C2004000639)

摘　　要：目的建立简单有效地获取大鼠肺微血管内皮细胞(PMVEC)的培养方法。方法从实验动物、培养基、植块方法、胎牛血清浓度等方面对植块培养法进行改进,应用W istar雄性大鼠的肺组织,进行PMVEC的原代培养并传代培养;通过倒置显微镜、扫描、透射电镜观察其形态,并进行免疫组织化学鉴定。结果体外培养的大鼠PMVEC呈梭形或多角形,形成单层后呈典型的鹅卵石样或铺路石样排列,获得的血管内皮细胞纯度较高,抗Ⅷ因子阳性反应,成功建立了PMVEC的原代培养方法。结论改进的植块培养法简便、可靠,获得的PMVEC纯度高,生长状态良好,保持了内皮细胞的结构和功能,可用于其功能特性的进一步研究。Objective To develop a simple and reproductable method for the isolation and primary culture of pulmonary microvascular endothelial cells（PMVECs） of rats. Methods The tissue block pasted culture method was improved in experiment animal, culture -medium, tissue block method, fetal bovine serum （FBS） and spreading slide method. Rats PMVECs were primary cultured with lung tissue block pasted methods using lung tissue of male Wistar rats, and the cell morphology was observed using inverted microscope and electron microscope, and identified using immunohistochemical methods. Results The cultured PMVECs of rats in vitro showed fusiform shape or polygon, and the monolayer cultures displayed a typical cobblestone or pavingstone morphology and were inhibited when contacted each other, the PMVECs expressed factor Ⅷ associated antigen. And the primary culture of PMVEC was established successfully. Conclusion The pure pulmonary MVECs of rats can be attained rapidly and easily by the described method. The method is simple, economic and reliable, availability of these cells will facilitate further functional character studies.

关 键 词：肺微血管 内皮细胞 细胞培养 

分 类 号：Q25[生物学—细胞生物学]