血管内皮生长因子对OX-LDL诱导的内皮细胞凋亡的影响及其机理  

The Effect of Vascular Endothelial Growth Factor on Vascular Endothelial Cell Apoptosis Induced by OX-LDL and its Mechanism

作　　者：程燕子[1] 刘启功[1] 廖德荣[1] 曾艳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,湖北武汉430030

出　　处：《中国微循环》2007年第1期20-22,26,共4页Journal of Chinese Microcirculation

基　　金：湖北省自然科学基金项目(NO.2003ABA135)

摘　　要：目的研究血管内皮生长因子(VEGF)对氧化型低密度脂蛋白(OX-LDL)诱导的血管内皮细胞凋亡的拮抗作用及其机理,以探讨VEGF预防血管成形术后再狭窄的机制。方法将对数生长期的人脐静脉内皮细胞分成对照组、OX-LDL处理组和OX-LDL+VEGF处理组,12 h后采用原位末端标记法和流式细胞术观察各组细胞的凋亡发生情况,并通过逆转录聚合酶链反应研究各组细胞中凋亡相关基因Bc l-2与Fas mRNA的表达情况。结果OX-LDL处理组凋亡细胞和Fas mRNA表达明显多于对照组和OX-LDL+VEGF处理组,而Bc l-2 mRNA表达情况相反。结论VEGF能部分拮抗OX-LDL诱导的血管内皮细胞凋亡,可能与其上调Bc l-2 mRNA表达及下调Fas mRNA表达有关,为VEGF预防血管成形术后再狭窄进一步提供了理论依据。Objective To study the inhibitory effect of vascular endothelial growth factor（VEGF） on vascular endothelial cell apoptosis induced by oxidized low density lipoprotein（OX-LDL） and its mechanism, and to evaluate the mechanism of VEGF on prevention of restenosis after angioplasty. Methods Human umbilical vein endothelial ceils（HUVEC） were divided into control group, OX-LDL-treated group and OX-LDL ＋ VEGF-treated group, the apoptosis of HUVEC was determined by flow cytometry（FCM） and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling（TUNEL）, the expression of Bcl-2 mRNA and Fas mRNA were examined by reverse transcription polymerase chain reaction（RT-PCR）. Results Compared with control group and OX-LDL ＋ VEGF-treated group, the apoptosis ceil and the expression of Fas mRNA of OX-LDL- treated group were significantly increased. However, the expression of Bcl-2 mRNA was markedly decreased. Condusion VEGF could inhibit the apoptosis of HUVEC induced by OX-LDL, which may correlated with upregulation Bcl-2 mRNA expression and inhibiting Fas mRNA expression, which offered further theoretical evidence for VEGF on prevention of restenosis after angioplasty.

关 键 词：血管内皮生长因子 细胞凋亡 氧化型低密度脂蛋白 血管内皮细胞 

分 类 号：Q25[生物学—细胞生物学]