药用植物墨旱莲的组织培养研究  

Tissue Culture of Eclipta prostrata L.

作　　者：韩丽[1] 郭顺星[2] 常明昌[1] 

机构地区：[1]山西农业大学,山西太谷030801 [2]中国医学科学院-中国协和医科大学药用植物研究所,北京100094

出　　处：《中国药学杂志》2007年第2期94-98,共5页Chinese Pharmaceutical Journal

基　　金：国家杰出青年科学基金资助项目(30325047)

摘　　要：目的研究利用组织培养技术对药用植物墨旱莲进行快速繁殖。方法以顶芽、茎、叶和幼苗(上部为绿体,下部为愈伤组织)为外植体,诱导愈伤组织及根和芽的分化。结果在顶芽、茎、叶和幼苗4种外植体中,顶芽的愈伤组织诱导率和芽分化率均高达100%,愈伤组织褐化少,生长良好,易被诱导出不定芽,是较为理想的快速繁殖材料;茎段的愈伤组织诱导率也达100%,但其中不带茎节的茎段不能直接诱导芽分化。在适宜的培养基中顶芽和带节的茎段能大量生根,生根率均达100%。结论较适宜诱导愈伤组织的激素组合是MS+6-BA2.0mg.L-1+NAA0.5mg.L-1,诱导芽分化适宜的激素组合为MS+6-BA3.0mg.L-1+NAA0.3mg.L-1,根的诱导则在MS+NAA0.2mg.L-1上进行。OBJECTIVE To study the speeding propagation of medicinal plant Eclipta prostrata L. by tissue culture. METHODS Bud, stem, leaf and seedling were used as explants to induce callus, adventitious roots and buds. RESULTS The results showed that bud was the best explant in speeding propagation among four explants. It had 100% inductivity and was easy to induce adventitious buds. Stem had also 100% inductivity of callus. The bud differentiation was induced when the stem without node was used as explant. The bud and stem with node produced many roots in appropriate media and the roofing percentage of two materials was up to 100%. CONCLU- SION The suitable plant growth regulator compositions to induce callus, adventitious buds and roots are MS ＋ 6-BA 2. 0 mg · L^-1 ＋ NAA 0. 5 mg·L^-1, MS ＋ 6-BA 3. 0 mg · L^-1 ＋ NAA 0. 3 mg · L^-1, MS ＋ NAA 0. 2 mg·L^-1, respectively.

关 键 词：墨旱莲 组织培养 再生 

分 类 号：Q942[生物学—植物学]