CdS纳米晶标记抗雌二醇抗体荧光免疫分析试剂  

Preparation of the Fluorescence Immunoassay Reagent of Estradiol by Using CdS Semiconductor Nanocrystals as Label

作　　者：王传涛[1] 王术皓[1] 杜凌云[1] 庄惠生[2] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059 [2]东华大学环境科学与工程学院,上海200051

出　　处：《河北师范大学学报（自然科学版）》2007年第2期218-221,共4页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金(20277005);山东省自然科学基金(2005ZX12)

摘　　要：在水相中制备了CdS纳米晶,其在536 nm处有较强荧光发射,用巯基乙酸修饰后与抗雌二醇抗体偶联,制备了CdS纳米晶标记的抗雌二醇抗体标记物,该标记物具有良好的荧光特性,发射波长红移至588 nm.利用该标记物竞争免疫分析法测定雌二醇的线性范围为0.20-8.00 mg/L.The CdS semiconductor nanoerystals was synthesis in water phase, and it has strong fluorescence emission at 536 nm after modified by mereaptoaeetie acid. The binding of the anti-estradiol antibody with CdS nanocrystals was prepared, and this binding had fluorescence characteristics. An competitive immunoassay method was developed for detecting estradiol by using the synthesized reagent, the linear range for determination of estradiol is 0.20-8.00 mg/L. The result showed that it could be used for determination of estradiol as fluorescence immunoassay reagent.

关 键 词：CDS纳米晶 荧光 抗雌二醇抗体 免疫分析试剂 

分 类 号：O657.3[理学—分析化学]