酶联免疫测定法(ELISA)在检测牛奶中氯霉素残留的应用  

Determination of Chloramphenicol Residues in Milk by ELISA

作　　者：王勇鑫[1] 宋燕青 宋杰[1] 王明费 赵宝华[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]石家庄市农村合作经济经营管理站,河北石家庄050019

出　　处：《河北师范大学学报（自然科学版）》2007年第2期252-255,共4页Journal of Hebei Normal University：Natural Science

基　　金：石家庄市科技攻关计划(03149312A)

摘　　要：运用酶联免疫法测定牛奶中的氯霉素残留.氯霉素在0.1-3μg/L范围内呈线性,线性方程为y=-22.306x+45.667,r=0.993 2.向样品中分别添加0.45,1.35,4.05μg/kg 3个水平的氯霉素,平均回收率分别为86.7%,89.6%和81.8%,最低检测限为0.1μg/L,批内变异系数为5.9%-8.4%,批间变异系数为5.9%-14.2%.结果表明该方法灵敏度高,重复性好,适合牛奶中氯霉素残留筛选检测.Chloramphenicol residues in milk were analyzed by ELISA. The calibration curve was linear in the range of 0.1 - 3 μg/L. The average recoveries were 84.5 %, 88.4 % and 79.9 % respectively when samples were spiked with 0.45,1.35 and 4.05μg/kg. The intra-assay coefficients of variation were 5.9 % -8.4 % and inter-assay coefficients of variation were 5.9 % - 14.2 %. The method is sensitive and suitable for the determination of trace chloramphenicol in aquatic products.

关 键 词：牛奶 氯霉素 酶联免疫法 

分 类 号：R978.13[医药卫生—药品]