新生大鼠心肌细胞原代培养方法的改进  

Improvement of Method for Primary Culture of New Natal Rats Cardiac Myocytes in Vitro

作　　者：王娜[1] 关鹏[1] 段相林[1] 李清[1] 常彦忠[1] 石振华[1] 

机构地区：[1]河北师范大学生命科学学院

出　　处：《河北师范大学学报（自然科学版）》2007年第2期256-259,263,共5页Journal of Hebei Normal University：Natural Science

基　　金：河北省自然科学基金(303158);河北师范大学博士基金(B2002010)

摘　　要：探讨更为简单的SD大鼠心肌细胞原代培养的方法,使分离的心肌细胞达到理想的存活率和纯度,为临床应用建立心肌细胞模型.取出生24 h内SD大鼠的左心室,剪碎、消化、分离和纯化,进行培养,0.1 g/L胰酶消化,可以分离到形态完整、贴壁生长的心肌细胞.进一步通过离心、差速贴壁和化学试剂抑制非心肌细胞生长,纯化得到95%以上的心肌细胞.成功建立了心肌细胞体外培养模型,获得了高纯度的心肌细胞.To discuss how to improve the primary culture method of rats＇ cardiac myocytes to increase the livability and purity of cells,left ventricle tissue was removed from postnatal 24 hours Sprague-dawley（SD） rats and then were used for primary culture after being removed, minced, trypsinized and purified. After digesting with 0.1 g/L trypsin, cardiac myocytes of integrity could be achieved and sticked to wall easily. By centrifugal selection, differential paces of sticking to wall and medical inhibit method, purification of cardiac myocytes reach to 95 %. Cardiac myocytes with high purity in vitro were successfully cultured.

关 键 词：新生大鼠 心肌细胞 原代培养 

分 类 号：Q813.11[生物学—生物工程]