硫酸盐还原菌类群分离培养和PCR分析鉴定  

Isolation Culture and PCR Identification of Sulfate-Reducing Bacteria Subgroups

作　　者：王明义[1] 梁小兵[1] 郑娅萍[2] 魏中青[1] 赵由之[1] 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵州贵阳550002 [2]贵阳医学院生物化学与分子生物学教研室,贵州贵阳550004

出　　处：《环境与职业医学》2007年第1期40-42,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:40473050;40173038)

摘　　要：[目的]探讨分离培养和聚合酶链反应(PCR)在硫酸盐还原菌类群分析鉴定中的应用。[方法]采用分离培养和PCR对贵州阿哈湖沉积物中硫酸盐还原菌类群进行分析,并对分离纯化的一株硫酸盐还原菌进行鉴定。[结果]贵州阿哈湖沉积物硫酸盐还原菌类群为脱硫肠菌属、脱硫叶菌属和脱硫球菌-脱硫线菌-脱硫八叠菌属;纯化菌株经PCR扩增初步鉴定为脱硫叶菌属,菌株形态与鉴定结果相符。[结论]采用分离培养结合PCR,可以对硫酸盐还原菌类群进行分析和鉴定。[ Objective ] To study the utilization of isolation culture and PCR in the identification of sulfate-reducing bacteria subgroups, [ Methods ] Isolation culture and PCR were performed to analyze sulfate-reducing bacteria subgroups in the sediment of Lake Aha in Guizhou Province and a purified strain identified from the sample, [ Results ] Desulfotomaculum, Desulfococcus- Desulfonema-Desulfosarcina and Desulfobulbus were detected in the sample, The purified strain was demonstrated preliminarily be Desulfohulbus and the morphologic characteristic of the strain is congruous with Desulfobulbus. [ Conclusion ] The analysis and identification of sulfate-reducing bacteria subgroups can be achieved by isolation culture and PCR,

关 键 词：分离培养 聚合酶链反应 硫酸盐还原菌 

分 类 号：R117[医药卫生—公共卫生与预防医学]