降解多氯联苯嗜盐菌的分离和降解特性  

Isolation and Degradation Characteristics of Polychlorinated Biphenyls-Degrading Halophilic Bacteria

作　　者：赵喆[1] 张兰英[1] 周佳欣[1] 

机构地区：[1]吉林大学环境与资源学院,长春130026

出　　处：《吉林大学学报（地球科学版）》2007年第2期380-383,共4页Journal of Jilin University：Earth Science Edition

基　　金：国家重点基础研究发展规划(973)项目(2004CB418505)

摘　　要：从深海底泥中提取出生长盐度在15％～20％的十二株嗜盐菌，对其进行分离、纯化和富集，进行了形态观察和革兰氏染色，最终选取了一株生长状态良好的菌株进行降解多氯联苯的影响实验。通过改变菌株降解PCBs的条件——pH值、接种量以及多氯联苯的浓度，得到降解多氯联苯的最适条件：在30℃下此菌株降解的最适pH值为7～8，最佳接种量为5mL，多氯联苯的浓度为3mg／L以下时，72h的降解率可以达到90％以上。Twelve halophilic bacterial strains were extracted from deep sea sludge, which could grow at the salinity between 15% and 20%. These halophilic bacterial strains were separated, purified and enriched. After morphological observation and dye identification, one strain was selected which grows very well to do the experiments of influence on degrading polychlorinated biphenyls at last. The optimal experimental conditions of PCBs-degrading were obtained according to the changes of pH, inoculation amount and PCBs concentration. The experiment indicated that the degradation ratio could reach to 90% when the temperature was 30℃, pH was 7 to 8, inoculation amount was 5 mL, the concentration of PCBs was below 3mg/L.

关 键 词：嗜盐菌 多氯联苯 生物降解 

分 类 号：X703[环境科学与工程—环境工程]