紫芝中三萜化合物标准化提取方法研究  

Optimized Preparation Method of Triterpenoids from Ganoderma sinense

作　　者：张宪民[1] 乔英[1] 邱明华[1] 

机构地区：[1]中国科学院昆明植物研究所植物化学西部植物资源持续利用国家重点实验室,昆明650204

出　　处：《天然产物研究与开发》2007年第1期109-112,共4页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向(KSCZX-SW-301-08);西部之光联合学者计划

摘　　要：为了更好地发挥紫芝的药用功效,本文优选了紫芝子实体三萜类化合物的提取条件。采用L9(34)正交实验设计,以提取溶媒、提取方法、提取时间和提取次数四因素三水平的提取工艺优选。各因素重要性依次为提取溶媒>提取次数>提取方法>提取时间。本文确定了以氯仿或丙酮为提取溶剂提取紫芝中三萜类物质的标准化方法。在提取过程中如果采用甲醇、乙醇或水作为提取溶媒,虽然提取效率高,但含糖、氨基酸等其它非三萜成分多,三萜类物质提取不全。The optimal preparation conditions of extracting triterpenoids of the fruiting bodies Ganoderma sinense were studied by L9（3^4） orthogonal experiments with respect to solvents ,time, methods and duration. The order in decreasing importance of these four factors is consistency 〉 times 〉 method 〉 duration. The results indicated that extraction of triterpenoids from Ganoderma sinense using chloroform and acetone was optimal. If methanol, ethanol and water were used as solvent in extraction process, compounds with strong polarity such as saccharides and amino acids would be the major extracted matter. However,triterpenoids in Ganoderma sinense are extracted incompletely even though the extraction efficiency is high.

关 键 词：紫芝 三萜 L9(3^4)正交提取试验 

分 类 号：R931.71[医药卫生—生药学] Q946[医药卫生—药学]