观光木叶片挥发油成分及其对超氧阴离子抑制与清除活性研究  

Study on Volatile Oil Components and Activities of Restraining and Eliminating Supper Oxygen Anion

作　　者：何开跃[1] 李晓储[2] 张双全[3] 樊英鑫[1] 邱金芬[1] 毕慧敏[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]江苏省林科院,江苏南京211153 [3]南京师范大学生命科学学院,江苏南京210097

出　　处：《林业科学研究》2007年第1期58-62,共5页Forest Research

基　　金：国家"十五"科技攻关项目"中国城市森林生态网络体系‘点’的研究与示范"(2001BA516A15-07);国家林业局科技项目"城镇绿化乔木树及生态功能树种区试"(2002-17A)的协作研究内容之一

摘　　要：用有机溶剂萃取法从观光木(Tsoongiodendron odorum)叶片中分离出挥发油,经GC/MS/计算机进行成分分析和定量测定,并且测定了其对超氧阴离子抑制与清除活性。鉴定出38种化合物,主要成分为酯类(33.396%),萜类(18.313%),醇类(12.065%)和芳香族化合物(9.484%)。研究结果表明,观光木叶片挥发油有较强的抑制与清除超氧阴离子活性。挥发油在稀释100倍时,抑制超氧阴离子活性最大,超过了植酸和没食子酸丙酯。在稀释20倍时,总SOD(T-SOD)活性达495.10 U.mL-1,其中CuZn-SOD活性为338.50 U.mL-1,占T-SOD的68.36%,Mn-SOD活性为156.70 U.mL-1,占T-SOD的31.64%。本研究结果为进一步探讨观光木叶片挥发油的保健功能提供了科学依据。The volatile oil from leaves of Tsoongiodendron odorum was isolated by organic solvent extraction and its components were analyzed and quantified by GC/MS/Computer. Meanwhile, its activities of restraining and eliminating ultra oxygen anion were detected. The 38 compounds in T. odorum were identified and the main constituents were ester （33. 396% ）, terpenoids （ 18. 313% ）, alcohol （ 12. 065% ） and aromatic compounds （9. 484% ）, The results showed that there were strong functions of restraining and eliminating ultra oxygen anion. When the volatile oil was diluted by 100 times , the activity of restraining ultra oxygen anion reached maximum, and its activity was stronger than that of PG and PA . When diluted 20 times, the activity of total SOD attained 495.1U·mL, among which the CuZn-SOD was 338.50 U·mL^-1, the 68.36% of T-SOD ; Mn-SOD was 156.70 U ·mL^-1, the 31.64% of T-SOD.

关 键 词：观光木 挥发油 化学成分 超氧阴离子 SOD 

分 类 号：TQ351.01[化学工程]