机械损伤对复叶槭叶片过氧化氢含量的影响  

Effect of Mechanical Wounding on the Content of Hydrogen Peroxide in Ashleaf Maple Seedlings

作　　者：张雯[1] 沈应柏[1] 沈瑗瑗[1] 

机构地区：[1]北京林业大学生命科学与技术学院,北京100083

出　　处：《林业科学研究》2007年第1期125-129,共5页Forest Research

基　　金：国家自然科学基金资助(30170764);北京林业大学研究生自选课题基金资助(05jj033)

摘　　要：以复叶槭为实验材料,采用DAB组织染色和分光光度法,定性定量地检测了人为机械损伤和外源茉莉酸甲酯处理0、15、30、60、120、240 min后损伤叶片,系统叶片以及诱导叶片内H2O2含量的变化情况。结果显示,机械损伤可诱导H2O2系统性产生,无论是受伤叶片、系统叶片还是诱导叶片,在伤害处理60 min内,H2O2含量一直增加,240 min后含量达最大值后开始下降。用1μmol.L-1茉莉酸甲酯熏蒸健康植株后,叶片H2O2含量显著增加。证明茉莉酸甲酯作为植株体内伤信号的报警信号分子,能代替机械损伤等外界刺激启动相似的信号防御系统,起到防御外界伤害的作用。Using ashleaf maple （Acer negundo） seedlings as materials, DAB tissue coloration and spectrophotometry as methods, the authors examined and compared the dynamic concentrations of H2O2 in different leaves： the damaged leaves, the intact leaves of the identical damaged plant and signal induced leaves of the neighboring plants damaged by 0,15,30,60,120,240 minutes. The results showed that the mechanical wounding could induce the systemic concentrations of H2O2, the contents sustaining increased in 60 minutes, and reached the max point at 240 minutes. After using the MeJA （1 μmol·L^-1）to treat the healthy plants, the concentrations of H2O2 increased distinctly. It could be concluded that MeJA induced the similar defensive systems instead of mechanical wounding, by way of initial signals in plants, and reached the effects of defending environment wound.

关 键 词：复叶槭 机械损伤 茉莉酸甲酯 诱导抗性 

分 类 号：S718.43[农业科学—林学]