HPLC法测定治糜灵栓中苦参碱和氧化苦参碱的含量  

Determination the Contents of Matrine and Oxymatrine in Zhimiling Suppositorys by HPLC

作　　者：李水清[1] 干国平[1] 刘焱文[1] 

机构地区：[1]湖北中医学院,武汉430061

出　　处：《中国药师》2007年第3期250-252,共3页China Pharmacist

摘　　要：目的:建立治糜灵栓中苦参碱和氧化苦参碱的HPLC含量测定方法。方法:采用Kromasil C_(18)柱(250mm×4.6mm,5μm),乙腈-无水乙醇-3%磷酸溶液(80:10:10)为流动相,检测波长220nm,流速1.0ml·min^(-1)。结果:苦参碱在0.17～0.86μg范围内,氧化苦参碱在0.50～2.50μg范围内,二者的峰面积与各自的浓度具良好的线性关系。平均加样回收率分别为100.4%(RSD=1.90%,n=6)和100.5%(RSD=1.23%,n=6)。结论:本方法简便、快速准确,可较好地控制该制剂的质量。Objective： To establish a HPLC method for determination the contents of matrine and oxymatrine in Zhimiling Suppository. Method： The Kroraasil C18 column （250 mm ×4.6 mm,5 μm） was used, the acetonitrile-ethanol absolute-3%-phosphoric acid solution（80： 10： 10） as raobile phase. The flow rate was 1.0 ml .min^-1 and the detection wavelength was 220 nra. Result： the linear ranges of matrine and oxyraatrine were at 0.17 - 0.86 μg（ r = 0.999 7 ） and 0.50 - 2.50 μg（ r = 0.999 8 ）, respectively. The average recoveries（ n =6） were 100.5% and 100.3% with RSD 1.9% and 1.2% , respectively. Conclusion： The method is simple, accurate and reproducible. It can be used for quality control of zhimiling suppositorys.

关 键 词：治糜灵栓 苦参碱 氧化苦参碱 HPLC 

分 类 号：R927.2[医药卫生—药学]