RP-HPLC法测定一枝黄花中槲皮素的含量  

Determination the Content of Quercetin in Herba Solidaginis by RP-HPLC

作　　者：尹雄章[1] 宋娅玲 黄泽中[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]武钢职工总医院中医科

出　　处：《中国药师》2007年第3期259-260,共2页China Pharmacist

摘　　要：目的:建立一枝黄花中槲皮素的含量测定方法。方法:采用HiQ sil C_(18)色谱柱(250mm×4.6mm,5μm);柱温:40℃;流动相:甲醇-0.1%磷酸溶液(50:50);流速:1.0ml·min^(-1);检测波长:371nm。结果:槲皮素进样量在0.40～4.04μg范围内线性关系良好(r=0.999 7),平均回收率为100.2%;RSD为1.7%(n=9)。结论:该含量测定方法简便准确。Objective： To establish a RP - HPLC method for determination the content of quercetin in herba solidaginis. Method： HiQ sil Cls column （250 mm× 4.6 mm,5 μm）was used, the column temperature at 40 ℃, the methanol- 0.1% phosphoric acid solution （50：50） as mobilephase. The flow rate was 1.0 ml.min^-1 and the detection wavelength was 371 nm. Result： The linear range of quercetin was 0.40 - 4.04 μg（ r = 0.999 7 ）. The average recovery was 100.2% with RSD 1.7% （ n = 9 ）. Conclusion. The method is simple and accurate.

关 键 词：一枝黄花 槲皮素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]