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作 者:吴建利[1] 施勇烽[1] 陈洁[1] Casiana VERA CRUZ 庄杰云[1] Hei LEUNG 郑康乐[1]
机构地区:[1]中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室,浙江杭州3100062 [2]国际水稻研究所昆虫和植物病理系
出 处:《中国水稻科学》2007年第2期131-135,共5页Chinese Journal of Rice Science
基 金:中央级科研院所科技基础性工作专项资助项目(2003DEB5J046);浙江省科技项目(2004C12032)
摘 要:The phenotypically uniform indica rice variety IR64 was chosen for study of the source and inheritance of within cultivar residual variation using a set of SSR markers.Molecular heterogeneity in IR64 was identified on the short arm of chromosome 2 involving at least five SSR loci spanning nearly 30 cM.The SSR variations originated from the parental lines of IR64(IR5657-33-2 /IR2061-465-1-5-5)and were segregating in the selfed bulk seed stock in a Mendelian manner for more than 20 years.This study was one of few examples to verify successfully that within cultivar variations of SSR in morphologically uniform selfing crops came from its parental lines,which has immediate and commercial applications including test of seed purity,varietal fingerprinting,and curation and propagation of germplasm collections.The phenotypically uniform indica rice variety IR64 was chosen for study of the source and inheritance of within cultivar residual variation using a set of SSR markers. Molecular heterogeneity in IR64 was identified on the short arm of chromosome 2 involving at least five SSR loci spanning nearly 30 cM. The SSR variations originated from the parental lines of IR64 (IR5657-33-2 / IR2061-465-1-5-5) and were segregating in the selfed bulk seed stock in a Mendelian manner for more than 20 years. This study was one of few examples to verify successfully that within cultivar variations of SSR in morphologically uniform selling crops came from its parental lines, which has immediate and commercial applications including test of seed purity, varietal fingerprinting, and curation and propagation of germplasm collections.
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