转ipt基因水稻种子萌发及苗期耐冷生理研究  

Study on the Seed Germination and Seedling Chilling-Tolerance of Transgenic Rice with Isopentenyl Transferase(ipt)

作　　者：段永波[1] 赵丰兰[2] 赵德刚[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业生物工程重点实验室,贵州贵阳550025

出　　处：《安徽农业科学》2007年第6期1591-1592,共2页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技攻关项目(黔科合[2002]1134);贵州省基金项目(黔基合计字[2002]3103)

摘　　要：采用28和15℃2种处理对转异戊烯基转移酶(ipt)基因水稻EY105株系7、株系19及EY105(CK)种子进行萌发,并对幼苗进行低温(4℃)胁迫试验。结果表明:常温下转基因水稻发芽势及发芽率高于对照,低温处理使转基因水稻与对照均受到抑制,但转基因水稻种子仍有较高发芽势及发芽率。在常温下,转基因水稻生理指标较CK差异不显著,低温处理后叶片可溶性糖含量、可溶性蛋白含量及根系活力均显著高于对照。The experiment in the stable expression of isopentenyl tmnsferase（ipt） in the transgenic rice EY105 line 7 （T7）, llne 19（T19） and the control EY105 were conducted through the seed germination and seedling chilling-tolerance under both normal temperature （28 ℃） and low temperature（15 ℃）. The results were as the follows： the transgenic rice can germ more efficient at normal temperature and low temperature, although both transgenic rice and control were restrained under low temperature. The physiological index measure didn＇t show the difference at normal temperature, but soluble sugar content, soluble protein content and mot activity were significantly increased under low temperature stress.

关 键 词：转ipt基因水稻 低温胁迫 

分 类 号：Q945.78[生物学—植物学]