斑点热群立克次体HL-93株ompA蛋白基因片段的克隆及序列分析  

Cloning and sequence analysis of genomic fragment encoding 190KD protein antigen of HL-93 spotted fever group Rickettsiae

作　　者：张健之[1] 范明远[1] 毕德增[1] 

机构地区：[1]中国预防医学科学院流行病学和微生物学研究所,北京102206

出　　处：《中华微生物学和免疫学杂志》1996年第3期227-230,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金;卫生部科研基金

摘　　要：以根据立氏立克次体分子量为１９０×１０＾３蛋白抗原基因序列设计的一对引物Ｒｒ１９０．７０ｐ和Ｒｒ１９０．６０２ｎ，用聚合酶链反应技术从斑点热群立克次体ＨＬ－９３株染色体ＤＮＡ中扩增出了１９０×１０＾３蛋白基因的部分片段。通过载体质粒ｐＧＥＭ－Ｔ将该部分基因片段克隆进入了大肠杆菌ＪＭ１０１，并进行了核苷酸序列分析。Genomic fragment encoding 190kD protein antigen was amplified from spotted fever group . ( SFG ) rickettsiae HL-93 strain by polymerase chain reaction with a pair of primers Rr 190. 70p and Rr 190. 602n which were designed from DNA sequences encoding 190kD protein antigen of R.rickettsii and cloned into E . coli JM 101 by using plasmid vector PGEM-T . The genomic fragment was sequenced and compared with that of R. rickettsii and R . Japonica which were reported previously. The results showed that 31 and 24 nucleotides in HL-93 strain differed from that in R. rickettsii and R. japonica , respectively. The genetic homology was 94% and 95. 5% respectively ,and amino acid homology was 87% and 89% respectively. All data suggest that HL-93 strain is a new member of SFG rickettsiae.

关 键 词：斑点热 立克次体 序列分析 HL-93株 ompA蛋白 

分 类 号：R376.3[医药卫生—病原生物学]