何首乌毛状根生物转化对苯二酚生产熊果苷的研究  

Studies on biotransformation of arbutin by 4-hydroxy phenol in hairy root of Polygonum multiflorum

作　　者：严春艳[1] 张章[2] 于荣敏[2] 孔令义[1] 

机构地区：[1]中国药科大学中药学院,江苏南京210009 [2]暨南大学药学院,广东广州510632

出　　处：《中国中药杂志》2007年第3期192-195,共4页China Journal of Chinese Materia Medica

基　　金：广东省自然科学基金资助项目(04010461)

摘　　要：目的:利用何首乌毛状根悬浮培养体系生物合成熊果苷,并对转化条件进行考察。方法:利用何首乌毛状根培养体系对底物对苯二酚进行生物转化,通过HPLC制定熊果苷标准曲线,以转化产物熊果苷的产量和转化率为指标,研究了共培养时间、底物加入浓度、培养瓶体积对生物合成熊果苷的影响。同时考察了产物在培养基中的分泌情况。结果:产物在培养物和培养基中同时存在。熊果苷标准曲线Y=440 740X?1.473(r=0.999 7)。当底物加入质量浓度为1 100 mg.L-1,共培养72 h时,熊果苷的产量(2.22 g.L-1)和转化率(81.45%)达到最优。3 L大瓶培养获得成功。结论:本研究大大提高了以生物转化方法生产熊果苷的产量和转化率,并达到了3 L大瓶培养规模。此外,本研究也为利用生物技术方法大规模生产熊果苷提供了有价值的参考资料。Objective： To study the biotransformation of arbutin by 4-hydroxy phenol in hairy root of Polygonum multiflorum. Method： 4-hydroxy phenol was used as substrate, the standard curve was made by HPLC, and the influences of the co-culture time, the concentration of substrate added and the volume of culture flasks on biotransformation of arbutin were measured by the index of the production yield and transform rate of arbutin. Result： Arbutin could be detected from both of the cultures and medium. The correlation curve of arbutin： Y=440 740X - 1. 473 （r =0. 999 7）. The production yield （2. 22 g· L^-1 ） and conversion ratio （81.45%） of arbutin reached the maximum amount as co-culture time at 72 h, substrate added in medium for 1 100 mg·L^-1. Furthermore a largescale culture of 3 L was also successful in our experiment. Conclusion： It was firstly to biosynthesis arbutin in hairy root of P. multiflorum. The production yield and trasfer rate of arbntin were increased largely. And large-scale production （3 L culture flask） of arbutin was achieved in the experiment and it would be valuable for the industrial production of arbutin by biotechnological method in the future.

关 键 词：何首乌 毛状根 生物转化 熊果苷 

分 类 号：S567.239[农业科学—中草药栽培]