冻干鲜鱼腥草超临界CO_2流体萃取工艺和提取物GC-MS分析  

Orthogonal experiment using SFE-CO_2 in extraction of essential oil from fresh Houttuynia cordata and analysis of essential oil by GC-MS

作　　者：孟江[1] 董小萍[2] 周毅生[3] 姜志宏[1] 梁士贤[1] 赵中振[1] 

机构地区：[1]香港浸会大学中医药学院 [2]成都中医药大学药学院,四川成都610081 [3]广东药学院中药学院,广东广州510000

出　　处：《中国中药杂志》2007年第3期215-217,共3页China Journal of Chinese Materia Medica

摘　　要：目的:优选超临界CO2流体萃取冻干鲜鱼腥草提取物的工艺,分析冻干鲜鱼腥草提取物的组成。方法:以甲基正壬酮含量为指标,采用正交试验法对超临界CO2流体萃取冻干鲜鱼腥草提取物的条件进行了优选,并用GC-MS法对提取物成分进行分析。结果:最佳萃取工艺:萃取压力15 000kPa,萃取温度35℃,萃取时间20 min。鉴定了冻干鲜鱼腥草提取物中出38种成分,并测定了其相对含量。结论:该工艺合理,能够有效保证提取物的质量。Objective： To optimize the extraction procedure of essential oil from H. cordata using the SFE-CO2 and analyze the chemical composition of the essential oil. Method： The extraction procedure of essential oil from fresh H. cordata was optimized with the orthogunal experiment. Essential oil of fresh H. cordata was analysed by GC-MS. Result： The optimize preparative procedure was as follow： essential oil ofH. cordata was extracted at a temperature of 35 ℃, pressure of 15 000 kPa for 20 min. 38 chemical components were identified and the relative contents were quantified. Conclusion： The optimum preparative procedure is reliable and can guarantee the quality of essential oil.

关 键 词：超临界CO2 流体萃取 冻干鲜鱼腥草 正交实验 GC—MS分析 

分 类 号：TQ461[化学工程—制药化工]