茉莉酸甲酯对丹参毛状根中丹参酮类成分积累和释放的影响  

Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root

作　　者：王学勇[1] 崔光红[1] 黄璐琦[1] 邱德有[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《中国中药杂志》2007年第4期300-302,共3页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(2006CB504700);国家高技术研究发展计划(2003AA2Z2040)

摘　　要：目的:研究茉莉酸甲酯(methy jasmonat,M J)对丹参酮类成分的积累和向培养基中的释放的影响。方法:6-7V悬浮振荡培养ATCC15834农杆菌诱导的丹参毛状根培养18d后,液体培养基中加入化学诱导子——茉莉酸甲酯,采用HPLC的方法,测定M J处理后不同时间段(2,6,9d)丹参毛状根中隐丹参酮、丹参酮IIA的含量以及其在培养基中的含量。结果:毛状根经M J处理后2,6,9d隐丹参酮的含量分别达0.039,0.204,0.572mg.g-1,分别是同时期未经M J处理的2.2,8.5,23.8倍;丹参酮IIA的含量达0.251,0.601,1.563mg.g-1,分别是同时间期未经M J处理丹参毛状根的1.9,4.1,6.2倍。结论:M J能显著促进丹参毛状根中丹参酮类成分的积累并向培养基中释放。Objective： To study the effects of methyl jasmonate （MJ） on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots. Method： After 18 day＇s suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents （including cryptotanshinone and tanshinone ⅡA ） on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC. Result： After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0. 039, 0. 204, 0. 571 mg· g^-1 respectively,and tanshinone ⅡA reached 0. 251, 0. 601 and 1. 563 mg· g^-1 respectively. After 9 day＇s treatment by MJ, the maximum increase of cryptotanshinone and tanshinone ⅡA were 23. 8 fold and 6. 2 fold higher than that of the control respectively. Condusion：＇ MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.

关 键 词：茉莉酸甲酯 丹参 毛状根 HPLC 

分 类 号：S567.53[农业科学—中草药栽培]