参苏饮含药血清对小鼠巨噬细胞Toll样受体及其下游信号转导通路的影响  

Effect of Shensuyin on the expression of toll-like receptors and the downstream signaling components on RAW 264.7 cells

作　　者：赵保胜[1] 李兰芳[1] 马悦颖[1] 郭淑英[1] 李沧海[1] 霍海如[1] 姜廷良[1] 

机构地区：[1]中国中医科学院中药研究所唐氏中药研究中心,北京100700

出　　处：《中国中药杂志》2007年第4期327-332,共6页China Journal of Chinese Materia Medica

摘　　要：目的:研究参苏饮含药血清对脂多糖(LPS)和聚肌胞(POLY I∶C)刺激的小鼠巨噬细胞株RAW264.7表达Toll样受体3(TLR3),Toll样受体4(TLR4)及其下游信号转导通路髓样分化蛋白(MyD88),肿瘤坏死因子受体相关因子-6(TRAF-6),Toll样受体相关分子(TRAM)和Toll样受体相关的干扰素活化子(TR IF)mRNA的影响。方法:用LPS(5μg.mL-1),POLY I∶C(50μg.mL-1)分别刺激RAW 264.7细胞株,同时给予参苏饮含药血清(11.2,6.7,4.0,2.4,1.5 g.kg-1.U-1)进行干预,ELISA法测定细胞培养上清液中炎症因子肿瘤坏死因子-α(TNF-α)和干扰素-β(IFN-β)的含量。先计算参苏饮IC50,然后再以IC50(11,5,2.5,4.9 g.kg-1.U-1)做为药物剂量进行干预,荧光定量PCR方法测定TLR3,TLR4和下游通路相关指标mRNA的表达,分析评价参苏饮含药血清清热解毒药效学作用的现代药理学基础。结果:参苏饮含药血清对POLY I∶C刺激的TLR3及其下游通路的MyD88和TRAM有抑制作用,对LPS刺激的TLR4的病理性升高无抑制作用,但对TLR4下游通路TRAM和TR IF mRNA的表达有明显的抑制作用。以上综合作用引起炎症因子TNF-α和IFN-β的降低。结论:参苏饮清热解毒的药效学作用可能与抑制TLR3,MyD88,TRAM和TR IF等细胞因子的表达有关。Objective： To investigate the influences of Shensu Yin to RAW 264. 7 on the expression of TLR3, TLR4 and the factors of the downstream in RAW 264.7 cells. Method： RAW 264. 7 cell line was stimulated with Lipopolysaccharide and POLY I： C,respectively, and treated with the drug serum of Shensuyin simultaneously. 24 hours later, collected the supernatant and measured the inflammatory factors TNF-α and IFN-β, extracted mRNA and measured the expression of TLR3, TLR4 and other correlated indexes of the downstream, analyzed and evaluated Shensu Yin＇s substance basis of pharmacodynamic actions. Result： Shensu Yin drug serum depressed the expression of TLR4, MyD88, TRAF-6, TRAM and TRIF mRNA, as a result, it decreased the amount of TNF-α and IFN-β. Conclusion： Depressing the expression of TLR3, MyD88, TRAM and TRIF mRNA may be the elementary basis of Shensu Yin to play heal-clearing and detoxicating effect.

关 键 词：参苏饮 含药血清 巨噬细胞 TOU样受体3(TLR3) Toll样受体4(TLR4) 

分 类 号：R285.5[医药卫生—中药学]